Regular aerobic physical activity (PA) increases exercise capacity and physical fitness (PF), which can lead to many health benefits. Accurate quantification of PA and PF becomes essential in terms of health outcome and effectiveness of intervention programmes. In this manuscript we present a review regarding the assessment of physical activity and fitness. Three types of PA assessment methods can be distinguished: criterion methods, objective methods and subjective methods. Criterion methods like doubly labelled water, indirect calorimetry and direct observation are the most reliable and valid measurements against which all other PA assessments methods should be validated, but they also hold important drawbacks. Objective PA assessment methods include activity monitors (pedometers and accelerometers) and heart rate monitoring. Finally, questionnaires and activity diaries are considered subjective methods. For the assessment of PF, we distinguish field tests and laboratory tests. The Eurofit for Adults is a test battery that is designed to assess health-related fitness of individuals, communities, sub-populations and populations. It is mainly used for evaluating the morphological component, the muscular component, the motor component and the cardio-respiratory component. In the laboratory, exercise capacity is preferentially assessed through maximal incremental exercise testing. Cardio-pulmonary exercise testing is a well-established procedure that provides a wealth of clinically diagnostic and prognostic information. The peak oxygen uptake is the gold standard in the assessment of exercise tolerance. When maximal exercise is contraindicated or not achievable, the VAT or the submaximal slopes provide reasonable alternatives.

Regular aerobic physical activity (PA) increases exercise capacity and physical fitness (PF), which can lead to many health benefits. Accurate quantification of PA and PF becomes essential in terms of health outcome and effectiveness of intervention programmes. In this manuscript we present a review regarding the assessment of physical activity and fitness. Three types of PA assessment methods can be distinguished: criterion methods, objective methods and subjective methods. Criterion methods like doubly labelled water, indirect calorimetry and direct observation are the most reliable and valid measurements against which all other PA assessments methods should be validated, but they also hold important drawbacks. Objective PA assessment methods include activity monitors (pedometers and accelerometers) and heart rate monitoring. Finally, questionnaires and activity diaries are considered subjective methods. For the assessment of PF, we distinguish field tests and laboratory tests. The Eurofit for Adults is a test battery that is designed to assess health-related fitness of individuals, communities, sub-populations and populations. It is mainly used for evaluating the morphological component, the muscular component, the motor component and the cardio-respiratory component. In the laboratory, exercise capacity is preferentially assessed through maximal incremental exercise testing. Cardio-pulmonary exercise testing is a well-established procedure that provides a wealth of clinically diagnostic and prognostic information. The peak oxygen uptake is the gold standard in the assessment of exercise tolerance. When maximal exercise is contraindicated or not achievable, the VAT or the submaximal slopes provide reasonable alternatives.

The enteric nervous system (ENS) is a network of neurons and glia within the wall of the gastrointestinal tract that is able to control many aspects of digestive function independently from the central nervous system. Enteric glial cells share several features with astrocytes and are closely associated with enteric neurons and their processes both within enteric ganglia, and along interconnecting fiber bundles. Similar to other parts of the nervous system, there is communication between enteric neurons and glia; enteric glial cells can detect neuronal activity and have the machinery to intermediate neurotransmission. However, due to the close contact between these two cell types and the particular characteristics of the gut wall, the recording of enteric glial cell activity in live imaging experiments, especially in the context of their interaction with neurons, is not straightforward. Most studies have used calcium imaging approaches to examine enteric glial cell activity but in many cases, it is difficult to distinguish whether observed transients arise from glial cells, or neuronal processes or varicosities in their vicinity. In this technical report, we describe a number of approaches to unravel the complex neuron-glia crosstalk in the ENS, focusing on the challenges and possibilities of live microscopic imaging in both animal models and human tissue samples.

We have configured a widefield fast imaging system that allows imaging at 1000 frames per second (512x512 pixels). The system was extended with custom processing tools including a time correlation method to facilitate the analysis of static subcellular compartments (e.g. neuronal varicosities) with enhanced contrast, as well as a dynamic intensity processing (DIP) algorithm that aids in data size reduction and fast visualization and interpretation of timing and directionality in neuronal circuits. This system, together with our custom developed processing tools enables efficient detection of fast physiological events, such as action potential dependent calcium steps. We show, using a specific blocker of nerve communication, that with this setup it is possible to discriminate between a pre and post synaptic event in an all optical way.

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