Purpose: Multiple sclerosis (MS) disrupts joint position sense (JPS). The aims were: - to assess the JPS of knee extensors, using three target angles in MS patients and in healthy; - to estimate the changes in reproducing the joint position by using absolute (AE), constant (CE) and variable error (VE) and the effect of sex on knee JPS acuity. Subjects and Methods: Twenty MS patients and twenty healthy were tested, by knee joint angle reproduction with three targets (20o, 60o, 75o), by passive and active mode, without visual and verbal feedback. JPS measurement was done using isokinetic dynamometry. Deviations from the targets were calculated as AE, VE and CE, which correspond to accuracy, precision and direction of the bias, respectively. Results: Increased values of AE and sex differences in JPS accuracy in MS individuals, as compared with healthy, were: - at targets 20o and 60o in females in passive mode in both legs; - at 60o in the involved leg in males (more pronounced as compared with females); and – at 75o in the less involved leg, in passive reproduction in women and active in men. The significantly increased negative CE indicates a more pronounced deficit in males with MS at 60o in the involved leg and at 75o in less involved leg. Increased VE in passive repositioning was also proved (p<0.05) in females with MS at 20o and 60o. Conclusion: These results suggest impaired proprioceptive performance and sex differences in MS individuals. The findings provide information to recommend appropriate strategies for the evaluation and treatment of proprioceptive deficits.