Diospyros anisandra is a small tree that contains metabolites with antitubercular, antibacterial, larvicidal, antioxidant, antihistaminic, and antiviral properties in its leaves, stem bark, and roots. However, this plant is slowly growing and takes about 10 years to reach maturity in situ. Therefore, it makes it an ideal model to apply tissue cultures and thus be able to have the metabolites of interest as required. In the present work we report for the first time, the establishment and comparison of three different protocols of in vitro culture of D. anisandra. This work aimed to establish stable in vitro cultivation conditions with seeds, leaves and nodal segments. They were cultured in Murashige and Skoog (MS) medium supplemented with plant growth regulators such as Gibberellic acid (GA3), zeatin, 6-benzylaminopurine (BAP), and 2,4-dichlorophenoxyacetic acid (2,4-D). The results showed that a germination efficiency of 73% was achieved in seeds with 57.8 µM GA3. In leaf regeneration, 80% regeneration of embryonic mass was achieved with 28.9 µM of BAP and zeatin. Finally, in nodal segments, an 85% efficiency in axillary shoot regeneration was achieved with 28.9 µM zeatin. On the other hand, the hexane extract of the plant segments of all treatments was analyzed by thin layer chromatography and gas chromatography-mass spectrometry (GC/MS). The main compounds detected are plumbagin, stigmasterol, β-sitosterol, taraxasterol, vitamin E, betulinic acid among others in the three systems studied, therefore, we can conclude that success was obtained in generating a cultivation method that allows us to have these compounds in less than 1 year of growth.

Global climate change caused by natural processes results in major environmental issues that affect the world. Climate variability results in changes that cause water stress in plants. Sugarcane is a tropical grass C4, perennial and a multipurpose industrial cash crop which serves as the main source of raw material for the production of sugar and biofuel. Farmers face the challenge to provide biotech alternatives with potential benefits and minimize potential adverse impacts on sugarcane's production. In order to find biotechnology strategies to diminish the impact of climate change, our laboratory teamworks with micropropagation, transcriptome and genetic transformation of sugarcane using the var. MEX69290. In the transcriptome of sugarcane, a total of 536 and 750 genes were differentially regulated under normal and water stress treatment respectively, of which key genes were selected to be inserted into sugarcane for tolerance to abiotic stress. Regarding results of micropropagation, it was concluded that the continuous immersion propagation system was the best culture strategy. This may be as result of the elimination of gelling agent, which additionally helps reduce production costs.