This paper presents the energy audit of a water network, which is obtained from the energy equation in integral form, and its time integration extended over a given period (day, month or year). The analysis allows accounting for all the energy in the system, showing that the energy balance is maintained. This balance allows can be used to obtain performance indicators to assess the system from the energetic point of view. From these indicators, it is possible to identify the improvement actions that will make the system more efficient. This energy audit requires a previous water balance and the mathematical model of the network, both of which are necessary to know the energy flows through the system's boundaries.

Fish larvae in aquaculture have high mortality rates due to pathogenic bacteria, especially the Vibrio species, and ineffective prophylactic strategies. Vaccination is not feasible in larvae and antibiotics have reduced efficacy against multidrug resistant bacteria. A novel approach to controlling Vibrio infections in aquaculture is needed. The potential of phage therapy to combat vibriosis in fish larvae production has not yet been examined. We describe the isolation and characterization of two bacteriophages capable of infecting pathogenic Vibrio and their application to prevent bacterial infection in fish larvae. Two groups of zebrafish larvae were infected with V. anguillarum (∼106 CFU mL−1) and one was later treated with a phage lysate (∼108 PFU mL−1). A third group was only added with phages. A fourth group received neither bacteria nor phages (fish control). Larvae mortality, after 72 h, in the infected and treated group was similar to normal levels and significantly lower than that of the infected but not treated group, indicating that phage treatment was effective. Thus, directly supplying phages to the culture water could be an effective and inexpensive approach toward reducing the negative impact of vibriosis in larviculture.

No abstract

No abstract