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A polyphenolic extract of Robinia pseudoacacia L. flowers was prepared using a hydro-ethanolic extraction phase. The chemical analysis of the extract consisted in the assessment of the total polyphenolic content (Folin-Ciocalteu method) and the flavonoid fingerprint of the extract determined by high-performance thin-layer chromatography (HPTLC). Two methods ferric reducing antioxidant power (FRAP) and radical scavenging activity with DPPH were used to measure the antioxidant activity of the extract. The cytotoxic and antitumor effects were evaluated on two cell lines: palatal mesenchymal stem cells (pMSCs) and epithelial cells derived from human cervical adenocarcinoma (HeLa (ATTC® CCL-2™)). The chemical content of Robinia pseudoacacia L. flowers extract reveals the presence of apigenin-7-glucoside and rutin. Results indicated high antioxidant activity with both methods (FRAP and DPPH). No cytotoxic effects of the extract were observed on the pMSCs cell line. The extract induced significant necrosis and apoptosis of the HeLa cells, proving an antitumor effect in vitro. The data confirm the antioxidant and antitumor effects of the polyphenols and the therapeutic potential of Robinia pseudoacaia flowers extract. In the last years, the therapeutic effect of the compounds from Robinia pseudoacacia flowers is less discussed; even the traditional medicine mentions them as helpful medicinal means. The present paper points out the correlation between Robinia pseudoacacia flowers polyphenolic compounds and their chemical and biological effects.

The fluorescence spectroscopy and in silico methods were used to evaluate the structural changes in bovine serum albumin (BSA) over a temperature range of 25–70 °C for 15 min. Experimental results indicated that the polypeptide chain rearrangements at temperatures higher than 40 °C lead to a lower exposure of hydrophobic residues causing the decrease in fluorescence intensity. The nonlinearity of the phase diagram indicated a sequential denaturation process involving several molecular species. The molecular dynamics simulations at the single‐molecule level showed the high stability of the BSA structure, compensating for the entropic costs associated with the prevalent helical folding.

The present paper describes a simple and efficient electrophoretic method to identify the species provenience of edible eggs in food products. The proteic pattern of egg yolk and egg white was described using polyacryl amide gel electrophoresis under denaturating conditions (PAGE-SDS) separately for the egg yolk and for the egg white proteins from five edible egg species, as follows: hen, goose, duck, turkey and quail. The molecular weight of each protein strip was calculated using a molecular weight standard curve. Separately, an electrophoretic protein pattern of all the mentioned samples was done using polyacryl amide gel electrophoresis under undenaturating conditions (native PAGE). The results show clearly distinct patterns in electrophoregrams resulted both in denaturating and undenaturating conditions for each species. These methods could be useful tools for egg species routine identification in various food industrial mixtures.