This article promotes an interviewing technique that could be used when interviewing elite policymaking respondents who fear repercussions for divulging information and who, as a result, either become too emotionally unstable to allow for rapport or begin to resist disclosing information. Based on two independent research projects in Bulgaria and Cyprus, the article advocates the active use of a new type of research participant, the intermediary. This relatively new interview participant is used to introduce and vouch for the credibility of the researcher. The paper argues their inclusion in the interview decreases a respondent's resistance by improving rapport and by preventing concealment of information. They achieve the former by: creating an aura of trust, by providing emotional support to the respondent and by converting the interview to a friendly conversation. They achieve the latter by intervening at the moments when they consider the respondent is deliberately or unintentionally withholding information.
Polyphenolic compounds, including total phenolics (TP), total anthocyanins (TA) and total flavan-3-ols (TF3-ols), as well as color intensity (CI) and hue (H), determined by spectrophotometric methods, were studied in Montenegrin red wines from Vitis vinifera L. cv, Vranac. Wines were produced by traditional and modern fermentation methods, applying different oenological products (enzyme, oak chips and grape tannins) during two vintages, i.e. 2008 and 2009. Wines produced from the 2009 vintage presented a slightly higher average amount of total phenols (2,878 mg/l) compared to the wines from the 2008 vintage (2,570 mg/l), and similar contents of anthocyanins and flavan-3-ols. The use of modern fermentation tanks (Sifa and Ganimede) followed by the addition of enzymes, oak chips and grape tannins resulted in a higher amount of polyphenolic compounds. Principal components analysis allowed for the grouping of wines according to the vintage and maceration method.
Aims: Eleven Macedonian grapevine accessions were genotyped by microsatellite profiling at 9 microsatellite loci, in order to identify Macedonian cultivars and to evaluate the relationships among them. The comparison with grapevine cultivars from two neighbouring countries was also performed.
Methods and results:Clustering analyses based on the proportion of shared alleles resulted in two clusters containing all accessions except cultivar « Vranec », which was distant from the others. Comparison of genotyping results of Macedonian accessions with 76 Bulgarian and 298 Greek accessions revealed no identical genotypes. In the dendogram, Macedonian accessions are dispersed among Greek and Bulgarian grapevines, suggesting a common genetic background. Additionaly, the synonyms « Smederevka » = « Dimyat » = « Zoumiatiko » and « Belo Zimsko » = « Karatsova Naousis » were also evaluated.
Conclusions:Clustering analyses showed that authentic Macedonian cultivars are distant from two widespread cultivars « Vranec » and « Smederevka ». Comparison of Macedonian cultivars with their synonyms from Greece and Bulgaria revealed differences in allelic profiles at some loci, but further analyses are needed to confirm their unique allelic profiles.Significance and impact of study: This work is a first step towards the genetic characterization of Macedonian grapevine germplasm, thus contributing to the molecular investigation of grapevine germplasm within the Balkan region. Conclusions : Les analyses de groupement ont montré que les cépages macédoniens authentiques sont éloignés des deux cépages répandus « Vranec » et « Smederevka ». La comparaison entre les cépages macédoniens avec leurs synonymes de Grèce et de Bulgarie ont révélé des différences dans les profils alléliques à plusieurs locus, mais d'autres analyses sont nécessaires pour confirmer leur profil allélique unique . Importance et impact de l'étude : Ce travail est une première étape vers la caractérisation génétique du germoplasme de la vigne de Macédoine contribuant ainsi à l'étude moléculaire du germoplasme de la vigne dans la région des Balkans.Keywords: Vitis vinifera, vigne macédonienne, génotype, microsatellite, synonyme
Abstract Résumémanuscript received the 15th of
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