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Lascufloxacin showed potent activity against with a GyrA or ParC mutation (first-step mutant). The frequency of selecting resistant strains tended to be lower for lascufloxacin than for levofloxacin and garenoxacin after drug exposure in first-step mutants but was similar in the comparison between lascufloxacin and moxifloxacin. The increase in MIC was smaller for lascufloxacin than for levofloxacin, garenoxacin, and moxifloxacin when clinical strains with only ParC mutations were exposed to the corresponding drug.

SUMMARY: Meropenem-susceptible and -resistant Aeromonas dhakensis isolates from blood cultures of a fatal case of septicemia were analyzed. The two isolates were homologous and gene expression of metallo-b-lactamase in the resistant strain was upregulated. Physicians should be aware of the possibility of the induction of carbapenem-resistance, following the use of carbapenems in the treatment of Aeromonas infection.In an earlier study, conducted at a hospital in Japan, we observed that Aeromonas septicemia occurred frequently in hospitalized patients with underlying diseases, including malignancy (1). In this report, a fatal case of Aeromonas septicemia in a girl with neutropenia is described. Two strains of Aeromonas veronii biovar sobria were isolated from blood cultures obtained before and after treatment with carbapenem. The strain isolated at the onset of sepsis was sensitive to carbapenem; however, the strain isolated after treatment, was resistant. Aeromonas species have chromosomal genes, such as cphA and imiS, encoding metallo-b-carbapenemase, which are not commonly expressed, but inducible. These genes have similar sequences and the enzymes can hydrolyze carbapenems efficiently (2,3). To understand the mechanism underlying the development of resistance to carbapenem, we analyzed the genetic homology between the blood isolates and the gene expression levels of the chromosomal metallo-blactamase.The two Aeromonas strains (meropenem-susceptible, MIC 0.5 mg/mL; meropenem-resistant, MIC

Simple and efficient screening methods of detecting carbapenemase producers are required. BD Phoenix CPO Detect panels effectively screened carbapenemase producers, particularly IMP producers, with a high overall PPA.