Introduction: Macroprolactinaemia is a well-known analytical problem in diagnostics of hyperprolactinaemia usually detected with polyethylene glycol (PEG) precipitation method. Since there is no harmonization in macroprolactin detection and reporting results, this study proposes and evaluates the usefulness of in-house developed algorithm. The aims were to determine the most suitable way of reporting results after PEG treatment and the possibilities of rationalizing the precipitation procedure. Materials and methods: This is a retrospective study based on extracted data for 1136 patients. Prolactin concentrations were measured before and after PEG precipitation on Roche cobas e601. Macroprolactinaemia was defined by percentage recovery and post-PEG prolactin concentrations. Results: Prevalence of macroprolactinaemia using recovery criteria of ≤ 40%, ≤ 60%, and post-PEG prolactin concentrations was 3.3%, 8.8% and 7.8%, respectively. Raising the cut-off value from the upper limit of the manufacturer’s reference interval to 32.9 μg/L does not drastically change detected macroprolactinaemia with recovery criteria. Post-PEG prolactin concentrations showed more than half of the patients with macroprolactinaemia would be overlooked. Regardless of the criteria, a cut-off of 47.0 μg/L would miss most of the macroprolactinaemic patients. Repeated recovery measurements of follow-up patients showed there is a significant difference with mean absolute bias of 9%. Conclusions: Post-PEG prolactin concentration with corresponding reference interval is the most suitable way of reporting results. All samples with prolactin concentration above the upper limit of the manufacturer’s reference interval should be submitted to PEG precipitation. Follow-up period could be prolonged since the difference between the recoveries of repeated measurements is not clinically significant.

Breast cancer is the most common malignant disease among women, constituting around a quarter of all cancers in women worldwide. This type of cancer is mainly affected by genetic, environmental, and lifestyle factors such as nutrition and physical activity. A retrospective study including 192 women with breast cancer was performed for six years (from 2015 to 2021). We investigated the relationship between indirect hematological parameters, neutrophil-to lymphocyte ratio – NLR, plateletsto-lymphocyte ratio – PLR, systemic immune-inflammation index – SII and the treatment outcome. Additionally, we also followed the overall survival (OS) rate. The obtained results report assessed parameters before and after surgical intervention. Of importance is to emphasize that at a cut-off value of 2.65 (P = 0.001) and 3.30 (P < 0.001), a decline in the NLR value was noticed after surgical removal of the breast cancer. The same decrease was observed for SII after surgery (P < 0.001). Through the study, SII has been shown to be a more relevant parameter compared to NLR and PLR. The study outcome recommends the cut-off value of 2.65 as the optimal for NLR in predicting the effectiveness and successfulness of the surgical procedure.

Introduction: The markers of inflammations are a significant predictor of postoperative outcome after colorectal cancer surgery. along with leukocytes, c-reactive protein (crp), procalcitonin (pct) and neutrophil/lymphocyte ratio (nlr) we tried to test the role of immature granulocytes (ig) counts and trends in the postoperative period. Methods: We recorded matched three patient according to their sex, age, and tumor localization who had an uneventful recovery with three patients who had an anastomotic leak required reoperation. We obtained the ig count with every complete blood count and correlated these with crp, pct, nlr. for the best prediction we calculated the potential Zagreb score by adding 1 point for elevated nlr ratio for more than 75%, minimally three consecutively elevated % ig, doubling of immature granulocytes relative value to absolute neutrophils count ratio (ig ratio) and immature granulocytes relative value to leucocytes count ratio (it ratio) at three consecutive time points. results and conclusion: potential Zagreb score seems to predict the need for reoperation in time. Herein we introduce its concept, and we plan the retrospective study to test its feasibility and precision.