The present study was undertaken to compare the anesthetic efficacy of tiletamine-zolazepam and ketamine-diazepam combination in 12 bitches presented to the TVCC, PGIVAS, Akola for ovariohysterectomies. These 12 dogs were randomly divided into two equal groups. Group I dogs were anaesthetized with tiletamine-zolazepam and Group II dogs were anaesthetized with ketaminediazepam combination. In both the groups xylazine was used as a pre-anaesthetic. Quality of anaesthesia, assessment of anaesthesia and clinico-physiological parameters were recorded at different time intervals during the study period. The quality of anaesthesia was found to be superior in group I as compared to group II. Induction of anaesthesia was rapid and smooth in both the groups.However, duration of anaesthesia and recovery time was longer in group I as compared to group II. Smooth recovery was observed in both the groups. Rectal temperature and respiration rate was showing decreasing trend in both the groups however, these values were within normal physiological range. Heart rate in both the group was showing decreasing trend with the appearance of bradycardia during recovery in both the groups. SpO 2 level was fluctuating within normal physiological limit in both the groups. Quality of muscle relaxation of tiletamine was better than ketamine and overall quality of anaesthesia of tiletamine-zolazepam was found to be superior as compare to ketamine-diazepam combination.