Lake Kanyaboli, an isolated satellite lake of Lake Victoria, has been suggested as a potential refugium for haplochromine cichlids that have gone extinct in the main basin of Lake Victoria. Mitochondrial DNA (mtDNA) molecular markers, as well as feeding ecology studies, were employed in this study to re-evaluate the evolutionary and ecological significance of six common Lake Kanyaboli haplochromines. The mtDNA marker revealed high genetic variability within four of the six haplochromine cichlids. Five haplotypes were discerned in Astatoreochromis alluaudi (n = 27), seven in Lipochromis maxillaris (n = 29), five in Astatotilapia nubila (n = 12) and 11 in the endangered Xystichromis phytophagus (n = 205). A haplotype genealogy suggests that Lake Kanyaboli harbours mtDNA haplotypes that could have been lost or not sampled in Lake Victoria, or could have arisen in situ. Lipochromis maxillaris appears to have undergone a recent demographic expansion. The pairwise F ST s indicated that only the comparison between X. phytophagus and A. nubila led to a nonsignificant F ST value. All other comparisons were significant at the 0.01 level, indicating the genetic distinctiveness of the haplochromines in the satellite lake. This could suggest that the lake harbours 'pure' relict populations of the haplochromines and therefore that Lake Kanyaboli can be considered a 'genetic reservoir'. Gut content analysis of the six haplochromine species revealed that eight different food items were consumed. No single species fed exclusively on a single food item, but certain food items contributed higher proportions of the fish diet for each fish species. Resource partitioning therefore could be discerned within this haplochromine community. Thus, Lake Kanyaboli and similar satellite lakes provide an opportunity for conservation of both genetic and trophic diversity threatened by introduction of exotics in the Lake Victoria basin. Lake Kanyaboli should be recognized and conserved as important evolutionary significant units for Lake Victoria region haplochromine species.
The common bean (Phaseolus vulgaris) is a high protein crop and the main legume in the cropping system of western Kenya. Despite its importance, common bean yields are low (<1.0 t/ha) and declining. Bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV) are the most common and most destructive viruses and can cause a yield loss as high as 100%. In Kenya, a limited number of cultivars and exotic genotypes with resistance to BCMV and BCMNV strains have been reported. This study sought to determine the distribution and screen popular cultivars for resistance to the viruses. In October 2016 and May 2017, two diagnostic surveys for bean common mosaic disease (BCMD) were conducted in seven counties of western Kenya namely Bungoma, Busia, Homa Bay, Nandi, Vihiga, Kakamega and Siaya. Leaf samples showing virus‐like symptoms were collected and analysed by ELISA. Sixteen popularly grown bean cultivars together with cowpea (Vigna unguiculata), soybean (Glycine max), green grams (Vigna radiata) and groundnut (Arachis hypogaea) were planted in a greenhouse in a completely randomized block design with three replicates. The plants were inoculated with BCMNV isolate at 3‐leaf stage. Data were taken weekly for 3 weeks on type of symptoms expressed and number of plants infected. In total, 270 bean farms were visited. Symptoms of mosaic, downward curling, local lesions, stunting or a combination of these were observed during both surveys. Mean virus incidence was higher in the short rain season (50.2%) than in the long rain season (35.6%). The mean BCMD severity on a scale of 0–3 was highest (2.3) in Kakamega County and lowest (0.5) in Siaya. On variety resistance tests to BCMNV isolate, 10 bean cultivars were susceptible, four tolerant and two resistant. BCMNV is widely distributed across counties probably because of use of uncertified seeds by farmers and inoculum pressure from seed and aphid vector. For improved yields of common bean, farmers should be advised to plant certified seeds for all legumes in the cropping system.
Tomato (Solanum lycopersicum) is one of the most important vegetable crop whose production involves the use of synthetic herbicides with detrimental impact on biodiversity. Allelopathy effectively controls horticultural crop weeds. Different plant parts (flowers, leaves, stems, bark, roots) have allelopathic activity that varies over a growing season when used as mulch. Mulching is a horticultural technique that protects the roots of plants from heat and cold by use of mulch to cover the soil surface around plants. Tomato production in Kakamega County is below 2% and weeds are ranked high among the yield reducing factors. This study assessed the allelopathic effect of guava leaves mulch type (18.0 cm thick) as a management tool for weed control in tomato crops and no mulch as control treatment with three most popular determinate tomato varieties. The mulch treatments were arranged as factorial in a Randomized Complete Block Design (RCBD) to minimize non–experimental bias. Tomato variety sub-treatments were replicated three times in the experimental plots at Masinde Muliro University of Science and Technology (00171N, 340451E). The field project was conducted during the short rains and long rains season of 2016-2017. Data obtained was subjected to analysis of variance (ANOVA) using SAS software, version 9.3 at p<0.05 confidence level. Least Significance Difference (LSD) was used to separate the means. Weed density incidence was significantly highest in control plots up to 100% and lowest in mulched plots (13.41%). Allelopathic control of weeds sustains global food and nutrition security for future generations.
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