The main aim of this study was to define the quantitative neuromuscular characteristics of the Biceps Femoris muscle (BF) as the knee joint flexor, i.e. the major synergist of the caudal body in all specific movement tasks of a football player, measured by the tensiomyography (TMG) method. The secondary aim of the study was to compare all TMG-BF characteristics in relation to bilateral dimorphism, as well as to compare dominant and non-dominant legs. The research was conducted on a sample which included 54 professional players aged 23.0 ± 4.4 years; body height: 182.6 ± 15.1 cm; body mass: 81.2 ± 15.1 kg; BMI: 23.3 ± 1.2 kg/cm 2 . TMG variables were measured on the muscles during a transition training phase (mid-season and end of the season). The results have shown that the average respectively. The results have indicated that there was no statistically significant difference between the examined TMG variables of the dominant and non-dominant leg (Wilks' lambda Value = 0.979, F=0.300, p=0.952, Part. Ƞ 2 = 0.021). It may be stated that there are no differences in the manifestation of neuromuscular characteristics in healthy elite football players regardless of their dominant leg. A high level of preparedness in football in addition to a completely healthy status of the body and locomotor system result in a complete neuromuscular contractile symmetry BF of both legs.