SummaryPseudomonas is one among the many bacteria that contribute to the deterioration of fish. Other microorganisms such as Salmonella sp., coagulase positive Staphylococcus and thermotolerant coliforms can also be found. The presence of this microbiota is always related to raw materials, poor handling, or badly designed plants. In this study the presence of these indicators was evaluated. The monitoring was conducted during the reception of the fish, chilling and storage of the product at À18C. As part of the monitoring, preventive measures for each Critical Control Point associated with the program of Hazard Analysis and Critical Control Point were established. The results showed that there was significant difference (p < 0.05) between the samples. The resistance of these microorganisms involved in getting the frozen fillets confirmed the need for more effective control and should be subject to a regulatory program like ISO 22000. This program specifies the requirements needed for a system that can monitor and master hazards and ensure that food is safe for human consumption.

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The objective of this study was to evaluate the acceptance of fermented anchovy (Engraulis anchoita) by consumers aged 18-67 (n=100) from two experiments, first using preference test and then by acceptance of formulation preference. Anchovy fillets were fermented with added NaCl and glucose with 4 different treatments: A (NaCl 1% and glucose 4%), B (NaCl 1% and glucose 6%), C (NaCl 1.5% and glucose 4%), and D (NaCl 1.5% and glucose 6% glucose). At first, the preference of fermented anchovy fillets with samples prepared in the form of pizza was assessed by applying a preference ranking test to 75 consumers. The results indicated the sample with 1% NaCl and 6% glucose as the preferred (P>0.05). Later in the second stage, the preferred fermented fillet was subjected to acceptance by 100 consumers who have the consumption habit of such product by using a hedonic scale of 9 points. The results indicated an acceptance rate of 79.8%. This work aimed to call attention to the importance of the acceptance of this food.

Lactobacillus sakei é comprovadamente uma cepa produtora de bacteriocinas e, neste estudo, procurou-se observar seus efeitos na fermentação de filés de sardinha (Sardinella brasiliensis) em diferentes concentrações de NaCl e glicose. Verificou-se sua habilidade para produzir ácidos orgânicos e conseqüente redução do pH durante 21 dias de fermentação. Ao término desse período, a concentração de microrganismos deterioradores atingiu 9,7 log UFC g -1 correspondente a 6% de NaCl e 4% de glicose. Poucas diferenças foram observadas na produção de ácido lático quando se adicionou 2 e 4% de glicose, já que a acidez total foi 1,32 e 1,34%, respectivamente, para os experimentos com 6% de NaCl. O pH 6 inicial dos filés modificou-se ao término de 21 dias para 3,8, 3,9 e 4 nos experimentos com 2, 4 e 6% de NaCl. Tal comportamento pode ser atribuído ao poder inibidor do NaCl sobre a microbiota deterioradora. No término da fermentação, a concentração de bactérias ácido lácticas foi 14,5 log 10 UFC g -1 . A relação entre o nitrogênio protéico e o nitrogênio solúvel total mostrou-se típica de pescado salgado/curado em perfeitas condições de consumo.PALAVRAS-CHAVE: FILÉS DE SARDINHA-FERMENTAÇÃO; PESCADO SALGADO; Lactobacillus sakei. *Professor Adjunto,

Lactobacillus sakei 2a is a bacteriocin producer strain and, in this work, it's effects as