No abstract

The aim of this study was to investigate the effects of paeoniflorin (PF) and paeonol (PN), the main active compounds of the Paeonia albiflora Pallas, on myocardial ischemia and reperfusion (I/R)-induced injury in Sprague-Dawley rats IN VIVO. Under anesthesia, the rats were subjected to 25 min of ischemia by ligation of the left anterior descending coronary artery (LAD) followed by 6 h (Western blot analysis) or 24 h (hemodynamics and infarct size) of reperfusion. When the infarct size was measured as the percentage of the area at risk, both PF (25.0 % +/- 7.0 %) and PN (24.1 % +/- 5.5 %) significantly (P < 0.05) reduced it compared to I/R control (54.8 % +/- 2.6 %). Administration of 10 mg/kg PF or PN 1 h prior to I/R injury also resulted in a significant improvement of the hemodynamic parameters. Furthermore, both PF and PN decreased the caspase-3 and Bax expressions but up-regulated Bcl-2 in the left ventricles. The results show that both PF and PN reduced myocardial damage in rat through protection from apoptosis, suggesting that Paeonia albiflora Pallas might be useful in treating myocardial infarction.

isolation and structure elucidation of a new megastigmane glucoside, simplicifloranoside (I) and three new flavonol glycosides, prunifolianosides A—C (II)

. As part of our efforts to isolate the chemical constituents of P. lactiflora for the purpose of its standardization, we isolated a number of major and minor constituents from a P. lactiflora extract. In the present investigation, we report the isolation and structure elucidation of the new minor compound 1, together with two known compounds, 1-O-b-d-glucopyranosylpaeonisuffrone and paeonidanin 1 ) (2) from P. lactiflora.

A new megastigmane glucoside, simplicifloranoside (1), and three new flavonol glycosides, prunifolianosides A–C (2–4, resp.), were isolated from the aerial parts of Spiraea prunifolia var. simpliciflora. In addition, fifteen known compounds, including five phenolic acids, three lignans, four flavonoids, one eugenol glycoside, and two alkyl‐primeverosides, were also identified. Their structures were elucidated on the basis of detailed spectroscopic analyses and acid hydrolysis.