Amicyanin, one of the type I copper proteins which has been used for the study, mediates the electron transfer reaction between methylamine dehydrogenase and cytochrome c-551i in Paracoccus denitrificans for energy production. The 69Histidine-tag site which has been widely used in purification of a recombinant protein was introduced at the N-terminus of amicyanin to make the complex of 69His-tagged plus cobalt functioning as a newly derived redox cofactor in amicyanin. In this study, Pro94 of amicyanin was substituted to Ala and Phe to tune up the midpoint potential (E m) value of amicyanin 100 mV more positive and then intra-electron transfer rates were measured to examine whether the E m value of the type 1 copper site in amicyanin affects intraprotein electron transfer or not. By the addition of H 2 O 2 , the Co 2?-loaded 69His-tagged site was activated, and then electron was transferred from Cu 1? of type 1 copper site of amicyanin to Co 3? plus 69His-tagged site. Electron transfer rates of cobalt loaded P94A and F amicyanin were much slower than that of native amicyanin. These results suggest that the communication between the newly protein-derived redox cofactor, 69His-tagged site plus cobalt, and type 1 copper site is truly occurred and that the strength of electron transfer reaction between them is able to be controlled by an E m value.

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The marine biotechnology industry is very significant as compared to other industries as one of the driving forces for economic growth in the next generation in Korea. However, the marine biotechnology market has been considered as a component of the biotechnology industry market which made difficult for creating separate research areas in relation to the scope of the relevant industry market as well as making it difficult to establish its own R&D policy strategies. Accordingly, this study was executed to estimate the future long-term market value of the marine biotechnology within the limit of industrial field and to verify the importance of national R&D investment in marine biotechnology on the basis of estimations within the industrial perspective. To this end, we classified the marine biotechnology industry into the four sub-sectors and estimated the domestic and global industrial market in 2010 and 2024. According to the results, the domestic and global market of the marine biotechnology industry will see a remarkable growth by 2024. In particular, the bio-energy, pharmaceutical and functional foods industry markets will achieve astonishing advances. On the basis of the analysis results, Korea has to establish more progressive and aggressive R&D investment strategies to strengthen national competitiveness through the marine biotechnology industry