This study was performed to evaluate the effects of conditioned media (CM) from human amniotic epithelial cells (HAECs) on the corneal wound healing process. Eighteen rabbits (36 eyes) were used and randomly assigned to three groups according treatment: CM from HAECs (group 1), vehicle alone (group 2), and saline (group 3). Corneal alkali injuries were induced with 1 N sodium hydroxide. Each reagent used for treatment evaluation was injected into the dorsal bulbar subconjunctiva and the area of the corneal epithelial defect was measured every other day. Two animals from each group were euthanized at a time on days 3, 7, and 15, and the cornea was removed for histological examination. The sum of the epithelial defect areas measured on day 0 to day 6 as well as day 0 to day 14 in group 1 was significantly smaller than those of other groups. Histological examination revealed that the group 1 corneas had less inflammatory cell infiltration and showed more intact epithelial features compared to the other groups. These results suggest that CM from HAECs promote corneal wound healing in rabbits.

Neuronal ceroid lipofuscinosis (NCL) is a group of inherited neurodegenerative lysosomal storage diseases in humans and animals and is characterized by apparently normal development followed by progressive deterioration in cognitive and motor functions, blindness, seizures, respiratory impairment, and premature death in most cases [1,2]. The diagnosis of NCL can be established through clinical signs, magnetic resonance imaging (MRI), histopathological findings, and gene sequencing used to probe mutated genes, including CLN10, CLN5, CLN12 and CLN8 [3,4]. However, gene sequencing is not generally conducted in veterinary medicine due to few studies on NCL diagnosis. Massive lysosomal accumulation in neurons and neuronal loss are reported to be representative pathological features of NCL [5]. Reported MRI lesions of the NCL include cerebral and cerebellar atrophy, mild hyperintensity of the cerebral white matter, cortical thinning, and thalami hypointensity on T2-weighted images [6]. Although several treatments, including enzyme replacement, stem cell and gene therapies, and pharmacological treatment, have been used to treat dogs with NCL, their prognosis is usually poor [7,8].A 2-year-old spayed female Border Collie presented with loss of vision, as evidenced by her bumping into walls, and progressive behavioral changes including aggression toward the owner. Decreased response to food from the age of 5 months and descending stairs incorrectly from the age of 1 year were identified through history taking. In addition, aggressive behavior of trying to bite the owner

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