No abstract

No abstract

Cloud Computing is the fundamental change happening in the field of Information Technology. It is a representation of a movement towards the intensive, large scale specialization. On the other hand, it brings about not only convenience and efficiency problems, but also great challenges in the field of data security and privacy protection. Currently, security has been regarded as one of the greatest problems in the development of CloudComputing. This paper describes the great requirements in Cloud Computing, security key technology, standard and regulation etc., and provides a Cloud Computing security framework. This paper argues that the changes in the above aspects will result in a technical revolution in the field of information security.

We report the first measured activation parameters for the additions of CCl2 and CClF to simple alkenes and demonstrate the existence of enthalpic barriers for CCl2 additions to cyclohexene and 1-hexene. With these two alkenes, additions of PhCCl are "dominated" by entropic contributions to deltaG++ and additions of CCl2 display comparable contributions of deltaH++ and deltaS++, while CClF additions feature dominant enthalpic contributions to deltaG++. Entropic factors, however, control the additions of all three carbenes to the highly reactive alkene, tetramethylethylene. Parallel computational studies are also reported.

The active site of lanosterol 14alpha-demethylase (CYP51) was investigated via MCSS functional group mapping and LUDI calculations. Several non-azole lead molecules were obtained by coupling structure-based de novo design with chemical synthesis and biological evaluation. All of the lead molecules exhibited a strong inhibitory effect on CYP51 of Candida albicans. They occupy the substrate-binding site and interfere with the binding of azole antifungal agents in a competitive manner. The mode of action of the lead molecules was validated by spectrophotomeric analysis and SAR studies. This is the first successful example reported for the inhibitor design of the cytochrome P450 superfamily using the de novo design strategy. Because the affinity of the lead molecules for CYP51 was mainly attributed to their nonbonding interaction with the apoprotein, the studies presented here afford the opportunity to develop novel antifungal agents that specifically interact with the residues in the active site and avoid the serious toxicity arising from coordination binding with the heme of mammalian P450s.