Sarcopenia is an age-related loss of skeletal muscle associated with adverse outcomes such as falls, fractures, disability, and increased mortality in older people and hospitalized patients. About half of older male nursing home residents have sarcopenia. The diagnostic criteria by the European Working Group on Sarcopenia in Older People (EWGSOP) and the Asian Working Group for Sarcopenia (AWGS) have led to increased interest in sarcopenia. Exercise and nutritional management are crucial for the prevention and treatment of sarcopenia. Nutritional therapy for sarcopenia that includes 20 g of whey protein and 800 IU of vitamin D twice a day improves lower limb strength. Exercise therapy for sarcopenia, such as resistance training and 6 months of home exercises, improves muscle strength and physical function. Combination therapy that includes both nutritional and exercise therapy improves gait speed and knee extension strength more than either exercise alone or nutrition therapy alone. Excessive bedrest and mismanagement of nutrition in medical facilities can lead to iatrogenic sarcopenia. Iatrogenic sarcopenia is sarcopenia caused by the activities of health care workers in health care facilities. Appropriate nutritional management and exercise programs through rehabilitation nutrition are important for prevention and treatment of iatrogenic sarcopenia. Nutritional and exercise therapy should be started very early after admission and adjusted to the level of inflammation and disease status. Repeated assessment, diagnosis, goal setting, interventions, and monitoring using the rehabilitation nutrition care process is important to maximize treatment effectiveness and improve patients' functional recovery and quality of life.

Abstract:This research was conducted with the goal of clarifying the required conditions of water-saving showerheads. In order to this, the research analyzes the mutual relationship between water usage flow, the level of satisfaction and the physical properties of spray of showerheads. The physical properties of spray were measured using physical properties test apparatus of standard or scheme for water-saving showerheads issued in several water-saving countries, and satisfaction evaluation data was acquired through bathing experiments. The evaluated showerheads were separated into three groups according to usage water flow and the level of satisfaction. The relationships between usage water flow, the level of satisfaction and physical properties were compared. The results identified that Spray Force and Spray Force-per-Hole were physical properties that influence usage water flow. Spray force-per-hole, water volume ratio in Spray Patterns within φ 100 and φ 150, Temperature Drop and Spray Angle were identified as physical properties that influenced the level of satisfaction. The level of satisfaction and usage water flow has a spurious correlation through the physical properties of Spray Force-per-Hole and Temperature Drop. It is possible to improve the level of satisfaction independent of amount of water usage through designs that set an appropriate value for water volume ratio and Spray Angle for Spray Patterns within φ 100 and φ 150.

tRNA m2G10/m22G10 methyltransferase (archaeal Trm11) methylates the 2-amino group in guanosine at position 10 in tRNA and forms N2,N2-dimethylguanosine (m22G10) via N2-methylguanosine (m2G10). We determined the complete sequence of tRNATrp, one of the substrate tRNAs for archaeal Trm11 from Thermococcus kodakarensis, a hyperthermophilic archaeon. Liquid chromatography/mass spectrometry following enzymatic digestion of tRNATrp identified 15 types of modified nucleoside at 21 positions. Several modifications were found at novel positions in tRNA, including 2′-O-methylcytidine at position 6, 2-thiocytidine at position 17, 2′-O-methyluridine at position 20, 5,2′-O-dimethylcytidine at position 32, and 2′-O-methylguanosine at position 42. Furthermore, methylwyosine was found at position 37 in this tRNATrp, although 1-methylguanosine is generally found at this location in tRNATrp from other archaea. We constructed trm11 (Δtrm11) and some gene disruptant strains and compared their tRNATrp with that of the wild-type strain, which confirmed the absence of m22G10 and other corresponding modifications, respectively. The lack of 2-methylguanosine (m2G) at position 67 in the trm11 trm14 double disruptant strain suggested that this methylation is mediated by Trm14, which was previously identified as an m2G6 methyltransferase. The Δtrm11 strain grew poorly at 95°C, indicating that archaeal Trm11 is required for T. kodakarensis survival at high temperatures. The m22G10 modification might have effects on stabilization of tRNA and/or correct folding of tRNA at the high temperatures. Collectively, these results provide new clues to the function of modifications and the substrate specificities of modification enzymes in archaeal tRNA, enabling us to propose a strategy for tRNA stabilization of this archaeon at high temperatures. IMPORTANCE Thermococcus kodakarensis is a hyperthermophilic archaeon that can grow at 60 to 100°C. The sequence of tRNATrp from this archaeon was determined by liquid chromatography/mass spectrometry. Fifteen types of modified nucleoside were observed at 21 positions, including 5 modifications at novel positions; in addition, methylwyosine at position 37 was newly observed in an archaeal tRNATrp. The construction of trm11 (Δtrm11) and other gene disruptant strains confirmed the enzymes responsible for modifications in this tRNA. The lack of 2-methylguanosine (m2G) at position 67 in the trm11 trm14 double disruptant strain suggested that this position is methylated by Trm14, which was previously identified as an m2G6 methyltransferase. The Δtrm11 strain grew poorly at 95°C, indicating that archaeal Trm11 is required for T. kodakarensis survival at high temperatures.

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Abstract:The purpose of this study was to construct a scheme that makes it possible to compare the relationship between water usage, satisfaction, and physical properties in three countries. The physical properties of the shower were measured using physical properties testing apparatus of water-saving standard or scheme for showerheads issued in several water-saving countries and data for users satisfaction evaluation was acquired through bathing experiments. In this paper, we analyzed the result from Taiwanese and Vietnamese individuals to compare them to of Japanese subjects analyzed in the previous study. We compared the physical properties of showers assessed low in satisfaction by Taiwanese, Vietnamese and Japanese subjects. It was assumed that spray pattern tends to decrease satisfaction when the water volume ratio within 100 mm and 150 mm of a measuring device is located a 450 mm distance from the showerhead is low, and that, because all three countries showed the same value, it was imagined that there were no differences in the water volume ratio of high-satisfaction showerheads among three countries. On the other hand, the values of Spray Force-per-Hole, Temperature Drop, and Spray Angle were different among three countries. We speculated that these differences are affected respectively by ethnic differences in pain tolerance, thermoregulatory response and bathing habit.