Objective(s):Complement C3 has the potential to impact bone homeostasis. The purpose of this study is to determine the difference in serum Complement C3 levels between osteoporosis patients and non-osteoporosis patients, as well as how Complement C3 silencing impacts and works on osteoblast osteogenesis.Materials and Methods: 58 people who underwent dual energy X-ray bone mineral densit(BMD) measurement in The Second Affiliated Hospital of Zhejiang Chinese Medical University from June to September 2021 were analyzed retrospectively. According to the classification of BMD t value, the subjects were divided into 31 cases in osteoporosis group and 27 cases in non- osteoporosis group. Complement C3 levels were recorded. and compared between the two groups. Then, the Complement C3 knockdown lentivirus was used to transfect Raji. The Raji-osteoblast line MG63 co-culture system was constructed after the stable strain was screened. CCK-8 and flow cytometry were used to assess the proliferation and apoptosis of MG63, alkaline phosphatase (ALP) activity was used to evaluate the osteogenic ability of MG63, and RT-PCR was used to assess the expression level of osteoprotegerin（OPG）of MG63.Results:Complement C3 levels in osteoporosis patients' serum are elevated，the proliferation level and osteogenic ability of MG63 were improved in Complement C3 knockdown Raji-osteoblast MG63 co-culture system. Furthermore, OPG expression in MG63 was enhanced.Conclusion:To sum it up, the level of Complement C3 in the serum of osteoporosis patients is elevated.Knocking down Complement C3 boost osteoblast osteogenic ability, which may be achieved by controlling OPG / RANKL / RANK system.

Objective(s):Complement C3 has the potential to impact bone homeostasis. The purpose of this study is to determine the difference in serum Complement C3 levels between osteoporosis patients and non-osteoporosis patients, as well as how Complement C3 silencing impacts and works on osteoblast osteogenesis.Materials and Methods: 58 people who underwent dual energy X-ray bone mineral densit(BMD) measurement in The Second Affiliated Hospital of Zhejiang Chinese Medical University from June to September 2021 were analyzed retrospectively. According to the classification of BMD t value, the subjects were divided into 31 cases in osteoporosis group and 27 cases in non- osteoporosis group. Complement C3 levels were recorded. and compared between the two groups. Then, the Complement C3 knockdown lentivirus was used to transfect Raji. The Raji-osteoblast line MG63 co-culture system was constructed after the stable strain was screened. CCK-8 and flow cytometry were used to assess the proliferation and apoptosis of MG63, alkaline phosphatase (ALP) activity was used to evaluate the osteogenic ability of MG63, and RT-PCR was used to assess the expression level of osteoprotegerin（OPG）of MG63.Results:Complement C3 levels in osteoporosis patients' serum are elevated，the proliferation level and osteogenic ability of MG63 were improved in Complement C3 knockdown Raji-osteoblast MG63 co-culture system. Furthermore, OPG expression in MG63 was enhanced.Conclusion:To sum it up, the level of Complement C3 in the serum of osteoporosis patients is elevated.Knocking down Complement C3 boost osteoblast osteogenic ability, which may be achieved by controlling OPG / RANKL / RANK system.

Background Prevalence information is the first step in developing preventive procedures or health services. This study was conducted to systematically evaluate the epidemiology of osteoporotic fractures in Chinese elderly aged ≥ 60 years and to provide evidence-based evidence for the prevention and treatment of osteoporotic fractures. Methods We identified relevant studies by searching the literature published in PubMed, Web of Science, Cochrane Library, Embase, CNKI, Wanfang Data, and VIP databases from the establishment of the database until August 2022. We used a random-effects model to obtain prevalence estimates and identified sources of heterogeneity and comparisons of prevalence among different groups through subgroup analysis and sensitivity analysis. Results A total of 29 articles were included in this study, and the prevalence of osteoporosis fractures in elderly Chinese was high (18.9%). The prevalence has increased significantly over the past decade (from 13.2% in 2000–2010 to 22.7% in 2012–2022). The prevalence of osteoporosis is higher in women than in men (18.5% vs 14.3%) and increases with age. The northern region was higher than the southern region (20.3% vs 18.9%), and the spine, hip, and distal forearm were the most common sites of fracture. Conclusion The prevalence of osteoporotic fractures in the Chinese elderly is 18.9%, and timely prevention and treatment are necessary.

Background Osteoporotic fracture is a common skeletal disease in the elderly, characterized by high mortality and disability, high medical costs, and social burden. A timely understanding of the prevalence of osteoporotic fractures in the elderly can provide a corresponding reference basis for prevention and treatment. However, there is no meta-analysis to study the prevalence of osteoporotic fractures in the elderly (≥ 60 years old) in Chinese. So we plan to systematically evaluate the prevalence of osteoporotic fractures in the Chinese elderly. Methods We systematically searched PubMed, Web of Science, Cochrane Library, Embase, CNKI, Wan Fang Data, and VIP databases about relevant studies on the prevalence of osteoporotic fractures in the elderly in China. After screening and evaluating the quality of the literature, relevant data were extracted for statistical analysis. The source of heterogeneity and the comparison of prevalence between different groups were confirmed through subgroup analysis and sensitivity analysis. Results The prevalence of osteoporotic fractures in the elderly was 18.9% [95%CI (16.5%, 21.4%)]. Subgroup analysis results: The prevalence of osteoporotic fractures was 14.3% [95%CI (10.1%, 18.5%)] in men and 18.5% [95% CI (14.8%, 22.3%)] in women. The prevalence of osteoporotic fractures was 15.9% [95% CI (12.2%, 19.6%)] in 60–69 years old, 25.0% [95%CI (19.6%, 30.5%)] in 70–79 years old, and 35.6% [95% CI (27.9%, 43.4%) ] in ≥ 80 years old. The prevalence of osteoporotic fractures was 18.9% [95% CI (15.9%, 21.8%)] in the southern region and 20.3% [95% CI (12.2%, 28.3%)] in the northern part. The prevalence of osteoporotic fractures in Chinese elderly was 13.2% [95% CI (10.4%, 16.1%)] from 2000 to 2010 and 22.7% [95% CI (18.7%, 26.6%)] from 2012 to 2022. The prevalence of osteoporotic hip fracture in older people was 4.5% [95% CI (3.3%, 5.7%)]. Distal forearm osteoporotic fracture was 3.0% [95% CI (2.2%, 3.7%)]. The prevalence of osteoporotic spinal fractures was 11.6% [95% CI (9.8%, 13.4%)]. Conclusion The prevalence of osteoporotic fractures in the Chinese elderly is 18.9%, and timely prevention and treatment are necessary. Trial registration: The ID number registered on Prospero is CRD42023383566.