The immobilized particles were used to treat acid mine drainage (AMD) in the study, which owns the characteristics of serious pollution and high managing cost. The immobilized particles were prepared with sulfate reducing bacteria (SRB) and medical stones. In order to investigate the interactive influence of medical stones on the particle properties, the salt modification condition, content, and size of the medical stone were taken as the influential factors. At the same time, the removal rate of SO and Mn, the release of total irons (TFe) and chemical oxygen demand (COD) and pH value were taken as the response values in the experiment. On the basis of the orthogonal experimental research, a response surface model was established. The experimental analysis showed that the particles can get the best treatment effect, when using the salt-modified medical stone with the content of 15% and particle size of 200~300 mesh. At this time, the removal rates of Mn and SO in wastewater were 83.10 and 96.22%, respectively. The release contents of TFe and COD were 2.99 mg L and 1828.54 mg L, respectively, and the pH value was 7.05. Then, biological medical stone particles were prepared according to the optimal ratio in the response surface experiment. The adaptability of biomedical stone particles was studied at different concentrations of SO, Mn and pH value. The results showed that the high concentration of SO inhibited the metabolism of SRB, while Mn had a less effect. The biomedical stone particles could regulate pH value very well.

To solve the problems of high concentrations of Cr, SO and H in agate dyeing industrial wastewater and heavy pollution and high treating cost, single-factor and orthogonal experiments were conducted to determine the optimum particle size, the ratio of adsorbents dosing and hydraulic retention time based on peanut shells and scrap iron. Experiments, using five dynamic columns filled with the peanut shells, scrap iron and sulfate-reducing bacteria (SRB), were also conducted to determine the effect and mechanism of treating the wastewater. The results show that the best treatment effect was obtained when the diameter of peanut shells was 3 mesh, scrap iron being 60 mesh size, scrap iron and peanut shells with a ratio of 1:2, and hydraulic retention time being 24 h. By the comprehensive comparison of five groups of columns, the treating effect of column 4 was best, in which the removal rate of SO and Cr was 30.17% and 88.36% respectively before adding the microorganisms, and 25.34% and 99.31% respectively after adding the microorganisms. The average of chemical oxygen demand (COD) release quantity was 62.11 and 513.75 mg·L, and the average effluent pH was 7.09 and 7.93 before and after addition of microorganisms respectively. In conclusion, peanut shells, scrap iron and SRB had a certain synergistic effect on treating agate dyeing wastewater.