(1) Background: pancreatic cancer is one of the most serious cancers due to its rapid and inevitable fatality, which has been proved very difficult to treat, compared with many other common cancers. Thus, developing an effective therapeutic strategy, especially searching for potential drugs, is the focus of current research. The exact mechanism of rutin in pancreatic cancer remains unknown. (2) Method: three pancreatic cancer cell lines were used to study the anti-pancreatic cancer effect of rutin. The potent anti-proliferative, anti-migration and pro-apoptotic properties of rutin were uncovered by cell viability, a wound-healing migration assay, and a cell apoptosis assay. High-throughput sequencing technology was used to detect the change of miRNAs expression. Immunoblotting analysis was used to detect the expression of apoptotic proteins. (3) Results: CCK-8 and EDU assays revealed that rutin significantly inhibited pancreatic cancer cells’ proliferation (p < 0.05). A wound-healing assay showed that rutin significantly suppressed pancreatic cancer cells’ migration (p < 0.05). A flow cytometric assay showed that rutin could promote pancreatic cancer cells’ apoptosis. Intriguingly, rutin significantly upregulated miR-877-3p expression to repress the transcription of Bcl-2 and to induce pancreatic cancer cell apoptosis. Accordingly, rutin and miR-877-3p mimics could promote apoptotic protein expression. (4) Conclusions: our findings indicate that rutin plays an important role in anti-pancreatic cancer effects through a rutin-miR-877-3p-Bcl-2 axis and suggests a potential therapeutic strategy for pancreatic cancer.

It is important to select buckwheat varieties suitable for foraging and determining their best harvest time as increasing attention was paid to the forage value of buckwheat. Here, eight tartary buckwheat varieties were identified as suitable for forage based on their potential forage value through assaying the contents of ash, crude protein, crude fiber, crude fat, acid detergent fiber, neutral detergent fiber, nitrogen free extract, calcium, phosphorus, total flavonoids, and rutin in these tartary buckwheat varieties at flowering, pustulation, and mature stages, respectively. In addition, analysis of relative feed value (RFV), relative forage quality (RFQ), and principal component analysis (PCA) based on the assayed contents was applied for comprehensive evaluation of these tartary buckwheat varieties. Results showed that all the eight tartary buckwheat varieties possessed potential high forage value as their RFV is from 121.31% to 217.39% and RFQ from 117.26% to 224.54% at all three stages. In particular, both RFV and RFQ values of PS-07 reached the highest at the flowering stage among the eight tartary buckwheat varieties, followed by CQ-3 and EWPS. Accordingly, the comprehensive scoring of principal component values of PS-07 and CQ-3 are relatively higher at the flowering stage. Our research thus revealed that the eight tartary buckwheat varieties are all suitable for forage, and also provided an experimental basis for selecting the eight tartary buckwheat varieties harvested at different growth stages for livestock forage.