Whole plant soybean (WPS) is a kind of legume resource with characteristics of high nutrition, large biomass, and wide distribution. In the present study, we have investigated the feasibility and effects of gallic acid (GA) on WPS silage quality, nitrogen distribution, tannin content, and bacterial community. The 0.5 and 1% (fresh matter basis) GA were added into WPS for dynamic ensiling (days 3, 7, 14, and 30, respectively). The results showed that the WPS silage with GA addition significantly decreased pH value (6.16–5.38 at ensiling day 30), coliform bacteria count and butyric acid (65.3–62.0 g/kg dry matter at ensiling day 30), and amino nitrogen contents (259–88.2 g/kg total nitrogen at ensiling day 30) and promoted lactic acid (9.62–31.5 g/kg dry matter at ensiling day 30), acetic acid (24.1–85.6 g/kg dry matter at ensiling day 30), and tannin (total phenol and hydrolyzable tannin) contents. Additionally, the GA addition also contributed to the change of bacterial community, where Firmicutes and Lactobacillus were most abundant on phylum and genus levels, respectively. The above results suggested that GA additive applied in WPS silage was an effective strategy to protect nutrition and improve fermentation quality, and the 1% GA addition showed a better effect.

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Mulberry (Morus alba) leaves has performed well as a high-quality protein supplement for livestock and enriches the edible resources of livestock. However, the harvest of mulberry leaves is seasonal and occurs mainly during the rainy season in southeast China; therefore, humid and sultry weather causes serious losses of mulberry leaf biomass, which pose a challenge for the preservation of mulberry leaves. In this study, we used the silage fermentation method to preserve mulberry leaves and investigated the effects of citric acid on the silage quality of mulberry leaves. Mulberry leaves were ensiled with or without 1% citric acid and 2% citric acid. The chemical composition, protein fraction and microbial community of mulberry leaf silages were analyzed. The results showed that the silage treated with citric acid had a higher dry matter recovery and lactic acid content and a lower acetic acid content, non-protein nitrogen content and ammonia-N content; citric acid also inhibited the activities of carboxypeptidase and aminopeptidase. Moreover, citric acid increased Lactobacillus abundance in silages and decreased the abundance of undesired microorganisms, such as Enterobacter. In summary, the addition of citric acid improved the fermentation quality of mulberry leaf silages, with 2% citric acid being more effective than 1% citric acid.

The presence of undesirable microorganisms in silage always leads to poor fermentation quality and low aerobic stability. Pyroligneous acid (PA), a by-product of biochar production, is known to have strong antimicrobial and antioxidant activities. To investigate the effects of PA on fermentation characteristics, aerobic stability, and microbial communities, Napier grass was ensiled with or without 1 and 2% PA for 30 days and then aerobically stored for 5 days. The results showed that PA application decreased (P < 0.01) the pH value, ammonia nitrogen content, and number of undesirable microorganisms (coliform bacteria, yeasts, and molds) after 30 days of ensiling and 5 days of exposure to air. The temperature of the PA-treated group was stable during the 5-day aerobic test, which did not exceed room temperature more than 2°C. The addition of PA also enhanced the relative abundance of Lactobacillus and reduced that of Klebsiella and Kosakonia. The relative abundance of Candida was higher in PA-treated silage than in untreated silage. The addition of PA decreased the relative abundance of Kodamaea and increased that of Monascus after 5 days of exposure to air. The abundances of Cladosporium and Neurospora were relatively high in 2% PA-treated NG, while these genera were note observed in the control group. These results suggested that the addition of PA could improve fermentation characteristics and aerobic stability, and alter microbial communities of silage.