This study determined the physical and mechanical characteristics of fresh black fungus during the harvesting season to provide basic data for the development of mechanical equipment for black fungus harvesting and processing. We have conducted a comprehensive test of black fungus cultivars “Heishan”. The mono-factor separation force experiments of black fungus and black fungus virgulate medium were conducted. It was noted that the tension angle was an important factor affecting the separation force, which was mainly distributed between 1.06 and 3.65 N. Besides, the average value of Poisson’s ratio of black fungus was measured to be 0.445 in the tensile test of black fungus leaves using image recognition and analysis techniques, with a test error within 2.5%; and the average value of tensile elastic modulus and shear elastic modulus of black fungus leaves was 0.947 MPa and 0.327 MPa, respectively; we also found that the average tensile strength at the root of black fungus was not significantly different from that at the leaf, which was around 0.436 MPa. In addition, it was obtained that the height and thickness dimensions of black fungus in the picking season conformed to a normal distribution, and concentrated around 34.39mm and 0.92mm respectively.