We report here on in vitro acceptor and site specificity of recombinant ␣3-fucosyltransferase V (Fuc-TV) with 40 oligosaccharide acceptors. Gal1-4GlcNAc (LN) and GalNAc1-4GlcNAc (LDN) reacted rapidly; Gal1-3Glc-NAc (LNB) reacted moderately, and GlcNAc1-4GlcNAc (N,N-diacetyl-chitobiose) reacted slowly yet distinctly. In neutral and terminally ␣3-sialylated polylactosamines of i-type, the reducing end LN unit reacted rapidly and the distal (
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