Background: 6a and 3r are the most implicated germ lines in light chain amyloidosis. Results: Mutagenesis at N-terminal, loop 40 -60, and CDR3 regions affected 3r fibrillogenesis. Conclusion: Changes at residues 7, 48, and 91 increased fibril formation while changes at residues 8 and 40 reverted fibrillogenesis. Significance: Characterization of light chain germ lines helps to identify key regions implicated in amyloidosis.
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