Background: There is an ancient history that human beings are familiar with use of animals and plants for food, cloth, medicine, etc. In Ethiopia, many ethnic communities which are dispersed all over the country has been totally dependent on local traditional medicinal system for their health care. Thus, the aim of this study was to take an ethnozoological field survey among different ethnic groups that live in South Achefer district. In order to document the ethno zoological information about animal and their parts/ products prevalent among the people in South Achefer district, a study was carried out from September, 2019 to January, 2020.Methods: Data was collected through questionnaire, focus group discussion and semi-structured questionnaire with 64 purposively selected respondents. Result: Ethnozoological data was recorded local name of the animals, animal products used, mode of preparation and administration Based on the ethno zoological survey, a total of 30 animal species were used in 42 different medicinal purposes including cold, night blindness, cough, paralysis and rheumatism and for other religious/and spiritual purposes. Based on the ethno zoological survey, 11 mammals, 7 birds, 4 reptile, 5 arthropods, 2 fish, 1 annelids are used in traditional zootherauptics in the study area. Furthermore, the milk of goat used to relieved cough has the highest FL (92%) and elephant teeth has the lowest FL (22%) used to treat bad spirit.Discussion: The results showed that ethno zoological practices have been an important alternative medicinal practice for the people residing in the study area. So, there is an urgent need to properly document to keep a record of the ethno zoological knowledge of the area. It is hoped that this information will be useful for further research in the field of ethno zoology, ethno pharmacology and conservation point of view.