Background and Objective: A thorough study was designed with an objective of observing utility of calretinin as immune-histochemical marker for aganglionosis and for detection of ganglion cells in the inadequate biopies of affected areas for more accurate and better diagnosis of the disease.

Objective: This study was designed with an objective of observing Calretinin as Immunohistochemical marker for aganglionosis and for detection of ganglion cells in the affected areas for more accurate and better diagnosis of the disease.