No abstract

The Danakil Depression in northeastern Africa represents one of the harshest arid environments on Earth, yet two genera of fishes, Danakilia (Cichlidae) and Aphanius (Cyprinodontidae), share its sparse aquatic habitats. The evolutionary history of these fishes is investigated here in the context of genetic, geological and paleoenvironmental information. We collected samples from seven sites and assessed phylogeographic relationships using concatenated COI and cytb mtDNA genes. Danakilia morphospecies show low differentiation at mitochondrial markers, but variation is partitioned between a northern cluster containing D. dinicolai plus three undescribed riverine populations, and a southern cluster including two creek populations of D. franchettii separated by the hypersaline waters of Lake Afrera. Aphanius displayed four genetically distinct clades (A. stiassnyae in Lake Afrera; one distributed across the entire area; one in Lake Abaeded; and one in the Shukoray River), but without clear large-scale geographic structure. However, Danakil Aphanius are clearly differentiated from A. dispar sensu stricto from the Sinai Peninsula. Geological evidence suggests that after the Late Pleistocene closure of the Danakil-Red Sea connection, increased post-glacial groundwater availability caused the formation of a brackish paleo-lake flooding the entire region below the -50 m contour. Fish populations previously isolated in coastal oases during glaciation were able to mix in the paleo-lake. Subsequently, in a more arid phase starting ∼7300 BP, paleo-lake regression isolated fishes in separate drainages, triggering their still ongoing diversification.

Summary An exploratory survey to phenotypically characterize indigenous chicken populations was carried out in Metekel zone of Northwestern Ethiopia in April 2013. A total of 69 males and 244 females were sampled to record their qualitative and quantitative traits. Eight quantitative and 16 qualitative variables were measured. Sampling included three districts representing different agroecological zones. Coefficient of variation for quantitative variables ranged from 6.38 to 52.37 percent in male sample populations and 4.59–21.4 percent in females. The chi-square tests for plumage colour of the neck, ear lobe colour and skeletal variant type were highly significant (χ 2 < 0.05). The correct classification percentage from discriminant analysis was 93.73 and 98.41 percent for male and female sample populations, respectively, indicating the homogeneity of the chicken populations within districts. The stepwise discriminant analysis identified five variables for male and three variables for female sample populations, which had the highest discriminating power. Canonical analyses showed that differences in body measurements between indigenous chicken populations were highly significant (P <0.0001). The results obtained from on-farm performance evaluation indicated that the average age at first lay of hens, number of chicks weaned and mean number of eggs laid per bird per year were 5.5 months, 6.5, 50.1, respectively. This information will constitute the basis for further characterization and development of conservation strategies for indigenous chicken populations of Northwestern Ethiopia.

The pattern of morphometric differentiation among three populations of Tilapia (Oreochromis niloticus) sited in Koka, Ziway, and Langano lakes in the Ethiopian rift valley was examined. Morphometric differentiation was examined, compared and quantified by the use of twelve different body measurements in 391 specimens to generate baseline information for conservation and product improvement plans. The result revealed that comparisons of mean values of morphometric measurements in the three studied tilapia populations statistically significant differences (P<0.05). The Fulton’s condition factor (K) of Oreochromi niloticus samples collected from Lakes Koka, Ziway, and Langano was 1.48, 1.2, and 0.66, respectively. The Discriminant Function Analysis performed on seven variables led to a correct classification in 90.5 % of cases. Differences observed between samples in this study indicated that there is important morphometric variation between the studied tilapia populations. Plotting the two significant canonical discriminant functions separated the Lake Langano population from the other two.

Sex-reversal in Oreochromis niloticus is used to produce mono-sex males which are desired in aquaculture for better growth performances and population control. The present study was aimed at studying the effect of elevated fry rearing temperature on survival, sex ratios, and growth performances of O. niloticus populations of Lakes Chamo, Koka, and Ziway. Fries from five separate brood pairs of each population were either treated (T) in elevated fry rearing temperature of 36 ± 1°C or kept at room temperature of 24 ± 1°C as control (C) groups for 10 days (phase-I) and then grown in outdoor ponds for six months (phase-II). Survival rates in T groups (74.0 to 91.7%) were lower than that of C groups (83.3 to 97.0%) in all the three populations in phase-I but not in phase-II. Chamo population was better in survival rate (88.4% and 95.4% in Phase-I, and 95.4% and 95.3% in phase-II) than Koka population (85.8% and 91.3% in Phase-I, and 93.9% and 93.5% in phase-II) and Ziway population (80.2% and 88.6% in Phase-I and 90.2% and 91.9% in phase-II) both in T and C groups respectively. The Chamo population also attained significantly (p<0.05) higher mean final weight of 31.58 ± 6.78 g and 24.26 ± 6.67 g in T and C groups respectively, followed by that of Koka population with 21.70 ± 5.10 g and 18.83 ± 4.16 g while Ziway population with mean final weight of 17.49 ± 4.60 g and 16.81 ± 4.15 g was the least both in T and C groups. The overall sex ratios in T groups were skewed towards male but balanced in all C groups of each population.  Better growth was achieved only in T groups with higher male ratio than their corresponding C groups. The number of sensitive brooding pairs and the overall male ratio in T groups were higher in the Koka population (40%, 61.68%) than in Ziway (20%, 56.78%) and Chamo (20%, 56.39%) populations respectively. However, the sensitivity of sex reversal to elevated fry rearing temperature was wide between individuals within each population than across the three populations. Hence, sensitive individuals can be selected from the populations and their degree of sensitivity can be improved through continuous selection of progenies from temperature-sensitive parents.