Peatlands could become important foraging habitats, and their plant communities threatened, in areas with an overabundance of large herbivores. Peatland response to herbivore exclusion may vary widely according to abiotic conditions and associated species because of a strong minerotrophic gradient. We assessed the impact of white-tailed deer (Odocoileus virginianus Zimm.) on peatland vegetation using an exclosure experiment. A total of 53 pairs of exclosures and unprotected plots were set up in bogs (13 pairs), sedge fens (20), shrub fens (7), and laggs (13), and surveyed prior to exclosure construction as well as three, five, and eight years after. Vascular plant composition of exclosures diverged from that of unprotected plots through time only in shrub fens and laggs. Bryophytes remained constant in all habitats. On average, shrub cover was 30% higher in exclosures in all habitats after five years, whereas herb cover increased only in laggs, by 43%, after eight years. Reclassification of sites by pH showed deer exclusion promoted alpha diversity in low- and high-moderate rich fens (pH 5.3–6.8) and beta diversity in the latter as well as in rich fens (pH 6.3–7.5). Overall, our results suggest that conservation efforts in areas with overabundant large herbivores should target richer peatland habitats since they showed a higher resilience and fostered alpha and beta diversity.