Liwa Botanical Garden is located in West Lampung Regency which has an area of 86.6 hectares and is one of the conservation of ornamental plants that presents the flora of the Bukit Barisan Selatan National Park. One of the flagship collections of this botanical garden is a native orchid. Disease infection is still a major obstacle in the cultivation and development of native orchid potential at Liwa Botanical Garden. Based on the results of previous studies, it is known that several individual natural orchid collections from Liwa Botanical Garden showed symptoms of fungal disease infections. Disease infections can inhibit plant growth and resistance and reduce the aesthetic value periodically. Symptoms of infection include necrotic patches on the upper surface of the leaf. This research was conducted to determine the identification of diseases and efforts to protect native orchids against fungal infections at Liwa Botanical Garden through a collection of samples that showed symptoms of infection, analysis of disease symptoms, and analysis of disease resistance levels. The results showed that the response of the native orchid at Liwa Botanical Garden to fungal infections was showed symptoms of wilting in the leaves, namely in the orchids Eria sp., Dendrobium sp., and Cymbidium sp. turns black on the edge of the leaf, Thrixspermum sp. The type of orchid most commonly infected with fungus is Cymbidium sp. a total of 8 samples, and Thrixspermum sp. 3 samples, Eria sp. as many as 4 samples, Dendrobium sp. and Calanthe triplicata as many as 6 samples. The type of native orchid that is most susceptible to fungal infections is Dendrobium sp. and Eria sp. with disease intensity of 74.11% and 22.3%. The results of this activity are expected to be basic information in efforts to protect plants against diseases to support the application of conservation of native orchids on Liwa Botanical Garden.

Several types of orchids that are in great demand and cultivated by various groups are Phalaenopsis sp. and Dendrobium sp. Mycorrhizae are needed by orchid plants to complete their life cycle. This is because mycorrhizae are capable of symbiosis with the root system of the host plant so that the plant's capacity to absorb elements will increase. This study aims to determine the extent to which Ceratorhiza induction on Phalaenopsis amabilis and Dendrobium discolour orchids can suppress the symptoms of Odontoglossum ringspot virus (ORSV) infection. The method used in this study was a factorial completely randomized design (CRD) with 2 factors and 4 replications. The first factor is the type of orchid used, namely Phalaenopsis amabilis (A1) and Dendrobium discolour (A2), while the second factor is the type of administration of mycorrhizae, viruses, and mycorrhizal viruses. Observation of disease symptoms is done by looking at the symptoms that appear on leaves that have been infected with ORSV. The results showed that the symptoms that appeared in Phalaenopsis amabilis were in the form of mosaic, necrosis, leaf malformation, while in Dendrobium discolour were mosaic and necrotic. Ceratorhiza was not able to suppress the symptoms of disease infection because based on the results obtained; the treatment on orchids inoculated with mycorrhizae and viruses showed more severe symptoms compared to the treatment only inoculated with viruses increased response and Dendrobium discolours showed a decreased response in leaf length, width, and number. Meanwhile, in the root organ, Dendrobium discolour showed an increased response and Phalaenopsis amabilis showed a decreased response in root length and number of roots.

The popular cultivated orchid is Phalaenopsis sp. and Dendrobium sp. Mycorrhizae are very important for orchid plants to complete their life cycle. Mycorrhizae are capable of symbiosis with the root system of the host plant which can increase the absorption of these elements. The purpose of this study was to find out the symptoms of infection, the intensity of infectious diseases, the level of resistance to infection. The method used in this study was completely randomized design (CRD) with 2 factors and 4 iterations, the first factor was the type of orchid used Phalaenopsis amabilis (A1) and Dendrobium discolor (A2), and the second factor was the type of administration of mycorrhizal., Virus , and mycorrhizal virus. Observation of the symptoms of the disease is done by looking at the symptoms that appear on the leaves that have been infected with ORSV, while to determine the intensity of the disease and the level of resistance can use a predetermined formula. The results showed that the disease intensity in Phalaenopsis amabilis was more severe than in Dendrobium discolor, which was 40% on each leaf. The level of resistance in Dendrobium discolor is more resistant than Phalaenopsis amabilis, this indicates that Dendrobium discolor on average has a tolerant response. Ceratorhiza could not suppress the intensity of the disease, because from the results obtained, treatment of orchids inoculated with mycorrhiza and virus showed more severe symptoms than treatment only inoculated with virus. Ceratorhiza was unable to increase the resistance of Phalaenopsis amabilis and Dendrobium discolor, this was because treatment of orchids inoculated with mycorrhiza and virus showed a very simple response compared to treatmentonly inoculated with virus.