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In the present study, a polymeric powder, synthesised using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linker, was used as a highly selective sorbent in a molecularly imprinted solid-phase extraction cartridge to extract penicillin G from milk samples. Detection was performed using high performance liquid chromatography. The proposed method was used to determine penicillin G in the milk matrix in the range of 3-1000 ng/mL (limit of detection 2 ng/mL) with relative recoveries of 81-90% (relative standard deviation 2.14-4.35%). Finally, the calibrated method was used to quantify penicillin G residue in commercial pasteurised milk samples.

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