SummaryLarge and small starch granules were isolated and character ized from mature barley kernels with waxy endosperms. The large granules of any given waxy cultivar contained more amylose than the small granules of the same cultivar.It was also found that large granules contained a greater amount of long amylopectin B chains and had a lower fraction III: fraction II ratio, one of the structural characteristics of amylopectin, than the small granules in the same cultivar. Small granules showed a wider range of gelatinization and smaller heat of gelatinization by differential scanning calorimetry.