The overall objective of this study was to determine the pattern of beta-adrenergic receptors in bovine luteal membranes obtained from slaughtered and from conscious cattle on different days of the estrous cycle. Two approaches were used with respect to the CL collection. In the first approach, CL (n = 20) were obtained from animals slaughtered in a local abattoir. The day of the estrous cycle was estimated based on the morphology of luteal tissue and follicles present. Using 3H-dihydroalprenolol (3H-DHA) as a ligand, we were unable to find specific binding in any of the tissues examined. Therefore, for the second approach we decided to collect CL from mature heifers or cows under local anesthesia on Days 4 (n = 15), 8 (n = 7), 12 (n = 6), and 16 (n = 3) of the estrous cycle by means of colpotomy or by lumbar incision. This procedure was chosen to reduce the influence of stress factors before CL collection. In these samples, specific beta-adrenergic binding sites were found and they varied during the estrous cycle. Maximal binding (Bmax; fmol/mg of protein) was highest on Day 4 (178.3 +/- 15.2); it then decreased significantly (73.2 +/- 14.7, 40.1 +/- 5.5, 10.8 +/- 0.8) on Days 8, 12, and 16, respectively. The dissociation constant (Kd; nM), which followed the same pattern, was 52.0 +/- 14.4, 16.3 +/- 3.9, 14.9 +/- 3.2, and 7.6 +/- 4.0) on the same days, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

ZAVOD ZA FIZIKALNU MEDICINU I REHABILITACIJU "DR MIROSLAV ZOTOVIĆ" BANJA LUKA, REPUBLIKA SRPSKA, BOSNA I HERCEGOVINA, SLATINSKA 11 SAŽETAK: Uvod: Dijabetesna polineuropatija je najčešća mikrovaskularna komplikacija dijabetesa. Alfalipoinska kiselina (ALK) i fizikalna terapija se koriste u liječenju ovog progresivnog oboljenja. Cilj rada je bio ispitati efikasnost kombinovane primjene ALK i fizikalne terapije kod pacijenata sa dijabetesnom polineuropatijom i utvrditi da li dužina trajanja dijabetesa, dužina trajanja simptoma polineuropatije, nivo glikoregulacije, indeks tjelesne mase i izraženost simptoma polineuropatije prije terapije utiču na bolji terapijski odgovor. Istraživanje je obuhvatilo 95 pacijenta sa dijabetesnom polineuropatijom kojima je parenteralno aplikovana alfa-lipoinska kiselina u kombinaciji sa kineziterapijom, vakumkompresivnom terapijom, karbotretmanom i galvanskim kupkama. Za procjenu efikasnosti terapije korišteni su Ukupni skor simptoma neuropatije (Neuropathy Total Symptom Score-4-TSS-4) i Toronto klinički sistem bodovanja (Toronto Clinical Scoring Sistem-Toronto CSS). Nakon primjenjenih terapijskih procedura, vrijednosti i TSS-4 i Toronto CSS su se statistički značajno smanjile (p< 0,01). Dužina trajanja dijabetesa, dužina trajanja simptoma polineuropatije, nivo glikoregulacije i indeks tjelesne mase nisu statistički značajno korelirali sa stepenom smanjenja TSS-4 (p> 0,05), dok je nađena statistički značajna negativna korelacija između vrijednosti TSS-4 prije terapije i stepena njegovog smanjenja (p< 0,05). Kombinovana primjena alfa-lipoinske kiseline i fizikalne terapije je efikasna u smanjenju subjektivnih simptoma i znakova dijabetesne polineuropatije. Terapijski odgovor je bolji ukoliko su simptomi polineuropatije manje izraženi. Ključne riječi: dijabetesna polineuropatija, alfa-lipoinska kiselina, fizikalne procedure. Summary:Diabetic polyneuropathy is the most common microvascular complication of diabetes. Alpha-lipoic acid and physical therapy are used in the treatment of this progressive disease. Aim of this study was to assess the efficacy of combined use of alpha-lipoic acid and physical therapy in patients with diabetic polyneuropathy and identify the factors which lead to a better therapeutic response. The study included 95 patients with diabetic polyneuropathy who were parenterally treated with alpha-lipoic acid in combination with the kinesiotherapy, vacuum compression therapy, carbon dioxide therapy and galvanic baths. The Neuropathy Total Symptom Score-4-TSS-4 and the Toronto Clinical Scoring System (Toronto CSS) were used to evaluate the effectiveness of the therapy. After the applied therapeutic procedures, the values of both TSS-4 and Toronto CSS statistically significantly decreased (p <0.01). The duration of diabetes, duration of symptoms of polyneuropathy, the level of glycemic control and body mass index were not significantly correlated with the degree of reduction in TSS-4 (p> 0.05), while we found a statistically significant negative correlation between the v...

Background/Aim. The primary rehabilitation in the prosthetic phase after amputation of lower extremities is of great importance for the improvement of the activities of daily living (ADLs) of persons with amputation and their successful social reintegration. The aim of this study was to examine the influence of independent predictors (age, gender, duration of rehabilitation, time between the amputation and the mounting of the prosthesis) on the success of the primary rehabilitation in the prosthetic phase after amputation of lower extremities. Methods. This retrospective clinical study included patients who underwent the primary rehabilitation in the prosthetic phase at the Institute for Physical Medicine and Rehabilitation "Dr Miroslav Zotović", Banja Luka, in 2015. A total of 75 patients with unilateral transtibial amputation were included. Etiologically, these transtibial amputations occurred as a consequence of vascular complications of diabetes mellitus or peripheral occlusive arterial disease. Evaluation of the success of rehabilitation was performed at the end of the primary rehabilitation in the prosthetic phase and 3 months after the end of the treatment by means of K-levels classification system and Locomotor Capabilities Index (LCI) scale. Depending on the distribution of data, univariate and multivariate multiple regression analysis, post hoc Mann-Whitney test, Spearman's correlation coefficient and Wilcoxon test were used for statistical analysis. Statistical significance of the found differences was set at p < 0.05. Results. A total of 75 patients, 55 (73.33%) men and 20 (26.67%) women, were included in this clinical trial. Average age of all participants was 63.5 ± 9.06 years, 61.8 ± 9.34 years for males and 68.1 ± 6.4 for females (p < 0.01). Average duration of rehabilitation was 27.69 ± 7.39 days in men and 33.9 ± 6.89 days in women (p < 0.01). Male patients had better functional results compared to females obtained by all analysed outcome measures (p < 0.01). Younger patients achieved better results, with the degree of statistical significance ranging between p < 0.05 and p < 0.001. The time from the amputation to the mounting of prosthesis and the duration of rehabilitation had no influence on the rehabilitation outcome. Conclusion. The present study identified age and gender of patients as relevant independent predictors of the success of rehabilitation. Although it was initially expected, this clinical trial did not prove the importance of the time from the amputation to the start of the primary rehabilitation in the prosthetic phase. In the future research other independent predictive factors, such as comorbidities, first and foremost cardiovascular diseases, medication, laboratory parameters and mental status, should be taken into account.