The introduction of Escherichia coli into natural seawater results in a direct response from the natural microbial parasites and predators, which increase in number and rapidly destroy the alien coliform bacteria. In a predictable successional sequence, the decline in numbers of E. coli was followed by a reduction in the parasite and predator populations resulting from the absence of suitable host organisms and from predation by larger protozoa. E. coli numbers declined more rapidly in seawater taken from near a sewage outlet, because the parasites and predators were enriched by the constant availability of host organisms. A slower rate of decline of E. coli numbers in seawater samples taken further from the outlet was attributed to lower initial populations of parasites and predators. Two common marine predators of E. coli, a fruiting myxobacter (Polyangium) and a small amoeba (Vexillifera), have been characterized.

The potential for N2 fixation by free-living bacteria using straw as a source of energy was evaluated in three soils (one from Gunnedah and two from Cowra) representative of the wheat belt in New South Wales. All three soils had a history of straw incorporation. The abilities of the respective microbial populations to use a range of carbon sources, including potential products of decomposition of straw, was determined and compared with the size and composition of each population. Neutral to alkaline (pH 7.4) soil of high (51%) clay content from Gunnedah produced higher rates of nitrogenase activity with straw than more acid (pH 5.6) lower (17%) clay containing soil from Cowra (site B). Gunnedah soil also contained a larger population of N2-fixing bacteria which used a broader range of energy sources than soil from either Cowra site B or Cowra site W (pH 5.8, clay content 34%). There was little difference in the composition of the N2-fixing populations in each of the soils except that Azotobacter spp. were absent from the acid Cowra soils. It was concluded that the difference in behaviour of the respective N2-fixing populations was primarily due to the physical characteristics of the soil affecting the numbers and activities of diazotrophic microorganisms. In addition some soil environments failed to support specific organisms.

The effects of stubble management practices on straw decomposition (C02 production) and nitrogenase activity C2H2 reduction) were examined in situ on a black earth (pH 7.4, clay content 51%)) near Gunnedah in the wheat-belt of New South Walcs. Straw treatments were: (1) mulching (bladeploughed) or surface cultivation (scarification), (2) burning and cultivation, (3) no-tillage, and (4) incorporation (disc-ploughed). In 1979, the straw was mulched on the surface in treatment 1. When moisture was applied, preliminary measurements (1 980) showed that nitrogenase activity was highest in the incorporated treatment with less in the surface mulched and no-tillage treatments respectively. There was only a small amount of activity in the burnt treatment due to some straw remaining. In a longer-term study in 1985 and 1986 straw in treatment 1 was lightly mixed near the soil surface by scarification. Following moisture application, nitrogenase activity was significantly higher in the scarified treatment than in the incorporated treatment, indicating that depth of mixing of straw with soil was important. Nitrogenase activity in the no-tillage treatment was similar to that in the incorporated treatment, and there was substantially less activity in the burnt treatment. Production of CO2 was similar in the straw-retained treatments, but significantly lower in the burnt treatment. In a series of short-term assays throughout 1985, microbial activity from January to May 1985 decreased with falling soil temperature. With the increase in temperature from July to November 1985, there was no corresponding increase in activity. Despite changes in microbial activity throughout the year, there was little change in the numbers of N2-fixing bacteria in the 14-month period from February 1985 to March 1986, indicating stability in the potential for N2 fixation. Although surface-cultivation (scarification) of straw is apparently the most favourable for free-living N2 fixation, other factors such as erosion and disease control need to be considered in deciding which straw management practice is to be adopted.

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