RésuméL'objectif de ce travail est le développement d'un capteur électrochimique à base d'un film mince de mercure protégé par du fibrinogène (Fbg). La préparation, l'optimisation, la caractérisation et l'application des électrodes à film mince de mercure modifiées par du fibrinogène sont étudiées. L'électrode de travail est une électrode à pâte de carbone modifiée par simple adsorption du fibrinogène contenu dans une solution aqueuse en quantité adéquate. Le film de mercure est électrodéposé à des potentiels négatifs sur l'électrode modifiée. Les paramètres expérimentaux tels que la concentration du fibrinogène, le temps de déposition et le potentiel de préconcentration sont étudiés. Les performances de cette électrode sont ensuite évaluées pour la détermination simultanée du plomb et du cadmium en présence de différents surfactants tels que la gélatine, l'albumine, le SDS et le Tween 20. Les limites de détection obtenues sont de 5.10 Abstract Analytical application of fibrinogen-coated mercury film electrodes for the detection of lead and cadmium in water samplesThe aim of this study was the development of sensor based on fibrinogen (Fbg) coated mercury film electrodes at carbon paste electrode in the perspective of in-field stripping analysis of heavy metals in complex matrix. The preparation, optimization and characterization of coatings of Fibrinogen (Fbg) for the modification of thin mercury film electrodes and its application for the detection of trace metals have been described. The modified carbon paste electrode was prepared by a simple adsorption of fibrinogen from an aqueous solution containing an adequate quantity of fibrinogen. The Mercury film was then prepared by electrodeposition through the fibrinogen coating. The experimental parameters such as concentration of fibrinogen, deposition time and deposition potential were investigated. The performance of this electrode is evaluated in a presence of different surfactants such as gelatine, albumin, SDS and Tween 20, while lead and cadmium were employed as test metal ions. Using selected conditions the limits of detection were 5.10 for lead and cadmium, respectively. Since fibrinogen coated mercury film showed the best performance and increased efficiency against surfactants when comparing to the conventional mercury film, it was used for the analysis of trace metals in environmental samples of waters and industrial effluents.
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