Studies on dimensional changes incurred during preparation of tissue specimens for the transmission and scanning electron microscopes are reviewed, with emphasis on quantitative measurements pertinent to morphometry and three-dimensional reconstruction. The scope of the review includes fixation, dehydration, plastic embedment, critical-point drying, and freeze-drying. Recommendations are presented for monitoring dimensional changes; a strategy for the choice of method of specimen preparation is outlined.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.