The most prominent over-the-counter antipyretic-analgesic drug is paracetamol (n-Acetyl-Para-Amino-Phenol, APAP). This study attempted to examine whether allicin (AC) and/or Omega-3 fatty acids (O3FA) could protect rats from the liver damage induced by APAP. Seventy rats were randomly distributed into seven groups (n=10): Control (saline), AC group received allicin (10 mg/kg, PO), O3FA group given omega-3 (100 mg/kg, PO), APAP group given paracetamol (1000 mg/kg, PO single dose on the 27th day), AC+APAP group received AC (10mg/kg) for 30 days and then given single dose of paracetamol on the 27th day (1000 mg/kg, PO), O3FA+APAP group received O3FA (100 mg/kg) for 30 days and then given single dose of paracetamol on the 27th day (1000 mg/kg, PO), and AC+O3FA+APAP group received O3FA (100 mg/kg) and AC (10 mg/kg) for 30 days and then given single dose of paracetamol on the 27th day (1000 mg/kg, PO). APAP had a significant negative impact on haematological and serum biochemical markers suggested that hepatic injury occurred in response to the APAP exposure. Histopathological examination of liver sections confirmed this hepatic damage where hepatic denegeration and necrosis were evident after APAP treatment. Allicin and/or omega 3 fatty acids treatment restore hepatic tissue architecture after treatment. Thus, pre-treatment with AC and O3FA alone or in combination was effective to reduce hepatic injury in APAP-intoxicated rats.