To prevent nosocomial infection in patients, maintaining acceptable indoor air quality (IAQ) in medical centers (MCs) is crucial. The properties of IAQ in several medical centers were investigated in this study. The aim of this was to focus on some indoor air pollutants in different medical clinics. Models of air samples were measured inside several medical clinics in Basra province. The results of TVOC, CH2O, and PM2.5 were distributed between exceeding the standard limits and others within the standard. The results in the urban (center of Basra city) were within the legal limits for TVOC and CH2O, but the rural areas were above the legal limits in those areas. PM2.5 were above legal limits but urban sites had the highest. Healthcare workers and patients are exposed to a variety of chemicals that vary with the activities and products used. The presence and exposure of VOCs, CH2O, and PM2.5 may lead to health effects on the patient people. Variation between rural and urban sites in pollutants concentration was clear.