In this research, we synthesized the Fe3O4/TiO2/multiwall carbon nanotubes (MWCNTs) magnetic nanocomposites using water extract of Spinacia oleracea leaves, and the high performance of synthesized catalyst was confirmed by employing of it in the multicomponent reaction of reaction of isatoic anhydride, tert‐butylisocyanide, diamines, or hydroxyamines, electron‐deficient acetylenic ester, α‐haloketones, and Et3N in water at ambient temperature for the production of new spiropyrrolopyrrolizine compounds in high yields. This catalyst could be used several times in these reactions and have main role in the yield of product. The synthesized compounds have NH and OH group in their structure and, for this reason, have good antioxidant activity. Also, employing Gram‐positive and Gram‐negative bacteria in the disk diffusion procedure confirmed the some spiropyrrolopyrrolizine derivatives antimicrobial effect. The results showed that synthesized compounds prevented the bacterial growth. This used procedure for preparation of spiropyrrolopyrrolizine derivatives have some advantages such as low reaction time, product with high yields, and simple separation of catalyst and products.