An electrochemical chemical sensor for determination of uric acid (UA) with highly sensitive and widely working range was fabricated using zinc layered hydroxide-sodium dodecyl sulphate-propoxur (ZLH-SDS-PRO) nanocomposites modified with multiwall carbon nanotubes (MWCNT). Introduction of the ZLH-SDS-PRO as a conducting matrix has enhanced the conductivity of MWCNT. The ZLH-SDS-PRO/MWCNT morphology was characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM), meanwhile the electrochemical behaviour of UA and K3[Fe(CN)6] at ZLH-SDS-PRO/MWCNT paste electrode was studied by square wave voltammetry (SWV) and cyclic voltammetry (CV), respectively. Under the optimized experimental conditions, the electrode established linear plot for UA from 7.0 µmol/L to 0.7 mmol/L (R2 = 0.9920) and the LOD was calculated to be 4.28 µmol/L (S/N = 3). The fabricated electrochemical electrode was successfully applied to urine samples and exhibit excellent stability and reproducibility, which made it worthwhile to be explored for analytical applications.