Genus Scutellaria belongs to the family Lamiaceae and is known to possess different pharmacological properties. S. litwinowii is a species from this genus that is endemic to Iran. In this work, antioxidant activities of methanol, dichloromethane, and ethyl acetate extracts of S. litwinowii were evaluated using thiobarbituric acid reactive species (TBARS) and 2, 2 -diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. In addition, antimicrobial activities of the mentioned extracts were screened against standard strains of Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. The minimum inhibitory concentrations and minimum bactericidal concentrations were determined by broth microdilution method and tetrazolium chloride salt. The highest antioxidant index (AI) was observed from the methanolic extract in both TBARS (41.6% at 32 mg/ml) and DPPH (92.29% at 16 mg/ml) assays. The AI of the methanolic extract in DPPH assay was significantly higher than those of other extracts (p < 0.001). However, the AI of all extracts were significantly lower than those of the reference compounds, namely vitamin E and butylated hydroxytoluene (p < 0.001). The results of this investigation indicated that extracts obtained from the aerial parts of S. litwinowii possessed antioxidant and antimicrobial properties. Further research is required to identify the active phytochemicals responsible for these biological activities.