Background: Orthotic devices like insoles are widely used to improve performance. This study investigated whether immediate effects of using insoles with various wedges influence on plantar pressure measurements and comfort level during marching.Methods: Twenty five able-bodied males participated in this study voluntarily. Repeated measures analysis of variance test with a Bonferroni correction (P<0.0014) was used to compare dependent variables among nine insole wedge conditions.Results: The result of present study showed that the comfort level was non-significantly higher in the anterior and medial wedges compared to the posterior-lateral and anterior-lateral wedges during marching (P>0.0014). The peak of plantar pressure and impulse in the first to third metatarsal regions decreased in the anterior, medial and anterior-medial wedges compared to the lateral and posterior-lateral wedges (P<0.0014). In addition, a significant negative relation between the comfort level and plantar pressure measurements was observed in the second and third metatarsals and midfoot regions during marching (P<0.05).Conclusion: It seems that using anterior, medial and anterior-medial wedge for insole may have some advantage through increasing comfort sense and decreasing the risk of stress fracture injuries in the second and third metatarsal regions. These findings should be considered during designation of shoes or insoles for military environments.