Background: The prevalence of obesity has risen dramatically in past several decades. Hormonal and genetic factor are rarely the cause of childhood obesity. Because obese adult may suffer lifelong physical and emotional consequences, this imperative to discuss prevention with parents during well-child examinations. Objectives: Purpose of the study was to compare the Serum lipid profile of obese and non-obese males. Materials and methods: Twenty two male students were selected from AL-Jouf University. Their age ranged from19 to 29 years. They were divided into groups. One group (N=15) having more than 20% fat was considered as obese group, another group (N=7) was considered as non-obese group. Fasting blood samples were analyzed for blood cholesterol, triglycerides, low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C), using chemistry analyzer, BS-300 Chemistry Analyzer. Results: Of obese group, significant higher cholesterol and triglycerides were observed compared to non obese group. On the contrary, obese group had significant lower HDL-C concentration than the non-obese group. The adult obese has relatively larger changes in serum lipids at any given level of obesity. Conclusion: On the average, the more fat, the more likely an individual will be dyslipidemic and to express elements of the metabolic syndrome. Increased triglycerides level in obese impaired lipolysis which reduced the HDL-C concentrations.