This study aimed to investigate a potential list of variables that may have an impact on the saturation flow rate (SFR) associated with different turning movements at signalized intersections in Jordan. Direct visits to locations were conducted, and a video camera was used. Highway capacity manual standard procedure was followed to collect the necessary traffic data. Multiple linear regression was performed to classify the factors that impact the SFR and to find the optimal model to foretell the SFR. Results showed that turning radius, presence of camera enforcement, and the speed limit are the significant factors that influence SFR for shared left- and U-turning movements (LUTM) with R2 = 76.9%. Furthermore, the presence of camera enforcement, number of lanes, speed limit, city, traffic volume, and area type are the factors that impact SFR for through movements only (THMO) with R2 = 69.6%. Also, it was found that the SFR for LUTM is 1611 vehicles per hour per lane (VPHPL),which is less than the SFR for THMO that equals to 1840 VPHPL. Calibration and validation of SFR based on local conditions can improve the efficiency of infrastructure operation and planning activities because vehicles’ characteristics and drivers’ behavior change over time.