Recombinant coagulation factor VII (FVII) is used as a potential therapeutic intervention in hemophilia patients who produce antibodies against the coagulation factors. Mammalian cell lines provide low levels of expression, however, the Spodoptera frugiperda Sf9 cell line and baculovirus expression system are powerful systems for high-level expression of recombinant proteins, but due to the lack of endogenous vitamin K-dependent carboxylase, expression of functional FVII using this system is impossible. In the present study, we report a simple but versatile method to overcome the defect for high-level expression of the functional recombinant coagulation FVII in Sf9 cells. This method involves simultaneous expression of both human gamma-carboxylase (hGC) and human FVII genes in the host. It may be possible to express other vitamin K-dependent coagulation factors using this method in the future.

This paper explains the use of remote ultrasound vibration at the optimum position and frequencies to vibrate plates under welding, with the aim of initiating cavitation in the molten pool area. It has been shown in the literature that ultrasound cavitation changes microstructure morphology and refines the grain of the weld. In practice, the plates are excited through narrow-band high-power ultrasound transducers (HPUTs). Therefore, a theoretical investigation is carried out to identify the plate-mode shapes due to the ultrasound vibration aligned with the frequency bandwidth of HPUTs available in the marketplace. The effect of exciting the plate at different locations and frequencies is studied to find the optimum position and frequencies to achieve the maximum pressure at the area of the fusion zone. It was shown that applying the excitation from the side of the plate produces an order of 103 higher vibration displacement amplitude, compared with excitation from the corner. The forced vibration of cavitation and bursting time are studied to identify vibration amplitude and the time required to generate and implode cavities, hence specifying the vibration-assisted welding time. Thus, the proposed computational platform enables efficient multiparametric analysis of cavitation, initiated by remote ultrasound excitation, in the molten pool under welding.

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This paper explains producing a novel ultrasonic system to remove/prevent biofouling growth from wind turbines’ access ladders by means of producing local ultrasound cavitation. Using bespoke hardware, an array of high-power ultrasound transducers (HPUTS) and optimally synthesized signal types to remove/prevent biofouling growth from the ladder without violating the standard noise level in the sea is explained. This is a non-toxic and non-invasive solution to detach biofouling and prevent biofilm initiation on offshore structures. It is shown that the marinisation of the HPUT slightly shifts the main resonance frequency from 28.1 to 27.5 kHz. The vibration output from the HPUTs with different mounting systems showed that the transducer with the horn could vibrate the plate at 20 cm from the excitation point, with 300 pm, six times higher than the vibration output from the marinised HPUT. A transducer array and attachment are proposed to make the ultrasound noise below the standard underwater noise limits. The produced sound pressure level (SPL) and sound equivalent level (SEL) from the proposed ultrasonic system was measured. It was specified that the SPL came below 120 dB at 25 m from the excitation point and the SEL value below the 173 dB limit. Finally, the effectiveness of the marinised HPUTS on biofouling removal has been demonstrated with an in-situ measurement, and it was indicated that local biofouling removal could be achieved.