Multidrug resistance Escherichia coli have one of most common resistance in the world. A total of 50 blood samples from Mansoura University Hospitals were collected, isolated, and identified E. coli, by molecular and chemical characterization using the Vitek AST automated technique. The results demonstrated antibiotic sensitivity results showed resistance against ciprofloxacin and moxifloxacin 100%. Ampicillin 100% Ampicillin/Sulbactam (91.93%), Aztreonam (96.93), Cefazolin (72.45%), Ceftazidime (100%), Ceftriaxone (94.68%), Cefepime (96%), Ciprofloxacin (100%), Gentamicn (73%), Moxifloxacin (100%), Nitrofurantoin 83.33%, Piperacillin/Tazobactam (100%), Tobramycin (97.53%), Trimethoprim/Sulfamethoxazoleol (70%), Ticarcillin (100%), Ticarcillin/Cavulanic acid (100%). Laboratory cultures of some fluoroquinolone groups, such as ciprofloxacin, levofloxacin, ofloxacin and norfloxacin, were also found to be resistant to E. coli bacteria. Two strains of Escherichia coli were used, and the antibacterial activity of varying concentrations (25, 50, 75, and 100) of aqueous garlic extract and peppermint was tested against the two strains. The findings revealed that although mint had no effect at all, garlic had a complete effect in all concentrations.