Cucurbita Pepo (Pumpkin) is a plant which is traditionally used to treat a wide variety of diseases, and through scientific investigation most of the properties have been validated. However, more scientific data is needed to support the various health claims. The aim of this study was to determine the fatty acids (FA) and Triacylglycerols (TAG) composition, tocopherols content and to evaluate the antioxidant activity of eight pumpkin seeds oil cultivated in Algeria. The results indicated that pumpkin seeds were rich in oil (15.8-33.5%) and the major unsaturated fatty acids were linoleic acid (42.1-48.5%) followed by oleic acid (18.4-39.6%), while the main saturated fatty acids were palmitic (13.91-20.00%).Unsaturated FA showed a preference for the internal position. Linoleic and oleic acids occurred predominantly in the sn-2 position as generally found in vegetable oils. The main TAGs were triunsaturated GU 3 and monosaturated GSU 2 as majorities compounds. The tocopherol content of the oils ranged from 7.7 to 31.9 mg/kg of oil for ␣-tocopherol, from 39.3 to 155.1 mg/kg for (+␥)-tocopherol, and from 39.0 to 103.0 mg/kg for δ-tocopherol. The capacity that would be suitable for food and industrial applications, as well as high unsaturation FA and tocopherol content that could potentially improve the nutrition of human diets.The data of antioxidant power determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and phosphomolybdenum (PPM) complex methods show that the level of the antioxidant activity by two used assays was significantly compared to synthetic antioxidants. Also, it was demonstrated for the first time that the studied oils possessed a good antioxidant activity which may be associated with their alleged health benefits.
Date pits are another major date palm crops and low grade rejected, used locally in the management of diabetes and as animal feed ingredient or turned into non-caffeinated coffee by the Arabs although their richness with valuable bioactive and antioxidant compounds. In this study and for the first time, lipids, fatty acids profiles, total tocopherols and sterols of nine (9) Algerian date palm cultivars (Phoenix dactylifera L.) seed oil were investigated and their antioxidant activities were evaluated. Oil yield ranged from 4.86% to 6.7%. Gas liquid chromatography revealed that the oil fraction contained unsaturated fatty acids in majority with the preponderance of oleic acid (37.83-55.00%) followed by lauric and myristic acids (6.63-25.36% and 9.30-19.33%) respectively. The study of the unsaponifiable fraction revealed the presence of sterols and tocopherols. The sterols contents ranged from 4.7 to 8.45 mg/g DW. Whereas, the levels of total tocopherols ranged from 0.32 to 0.74 mg/DW, which ␣-tocopherol was the predominant tocopherol (15.57%-74.1%) followed by ( + ␥)-tocopherol (25.12%-56.01%). The antioxidant activity of date seeds oil was determined with DPPH radical scavenging assays. The oils had a moderate bleaching effect on DPPH free radicals, where Ghars (GH), Sbo3 Lossif (SL) and Timjhourt (TIM) seed oils were the active ones. The richest of date seed oil by active components such sterols, tocopherols also high amounts of oleic, lauric and other fatty acids suggest it to be as a useful source of edible oils for human consumption as well as using as additives in food, for medicinal use, in cosmetics, pharmaceuticals and food industries.
BACKGROUND: One of the objectives of food industry is to seek new resources of oil that preferably presents nutritional values. For human health, fats and particularly vegetable oils are considered as an important source of energy when glucose is not available. In addition, the search for effective, natural compounds with antioxidant activity has been intensified in recent years to replace the synthetic products. OBJECTIVE: The aim of the present work was to determine the fatty acids profile (and variability) of the lipid fractions extracted from five different populations of the leaves of Pistacia lentiscus L. growing in Algeria. In addition, the antioxidant activities of the lipidic fractions were also determined. METHODS: The chemical compositions of tocopherols were also analyzed by HPLC for the first time for this plant part. The chemical percentage variability (presence of two main distinguished clusters) of the fatty acids was discussed using statistical analysis methods (Agglomerative Hierarchical Clustering “AHC” and principal component analysis “PCA”). The antioxidant activity of the dewaxed lipid fractions were investigated in vitro using two different assays: DPPH (1,1-diphenyl, 2-picrylhydrazyl) free radicals scavenging activity and β-carotene bleaching test. RESULTS: For the overall samples, the main saturated fatty acids components were capric acid (C10:0 = 2.49–13.88%), myristic acid (C14:0 = 4.71–9.12%) and palmitic acid (C16:0 = 5.31–9.03%). Alternatively, the main unsaturated fatty acids were oleic acid (C18:1w9 = 3.42–4.85%), linoleic acid (C18:2w6 = 10.94–16.99%) and most importantly α-linolenic acid (C18:3w3 = 20.92–48.92%), which is known for its multiple dietary, pharmaceutical and clinical benefits. CONCLUSION: The results of fatty acids methyl esters identification using GC and GC-MS showed a clear variability in the composition of fatty acids. The main result of this study illustrate the nutritional potential (richness in MUFA such as C18:1w9, presence of essential fatty acids such as C18:2w6 at all the stages of maturation, richness in omega-3 fatty acids such as C18:3w3. of the oil of P. lentiscus leaves, which can provide opportunities for rational exploitation for medicinal purposes and in the food industries. Regarding tocopherols HPLC analysis, the samples were rich in α-tocopherol (58.51–89.17% of the total tocopherols identified). Finally, and for antioxidant activity measured by β-carotene assay, the obtained values suggested good antioxidant activities when compared with antioxidants of reference.
BACKGROUND: Margarine is a widely consumed product in Algeria. Few or no studies have been conducted to estimate its safety and nutritional quality. OBJECTIVES: This study aims to evaluate some algerian margarines. Particular interest is given to their oxidative stability and fatty acid composition. METHODS: Twelve margarines are selected, including tub and stick margarines, puff pastry margarines and vegetable smen. We evaluate physicochemical parameters (water content, pH, salt content, melting point and solids content with RMN), oxydative stability with Rancimat and fatty acid composition with GLC. RESULTS: The analyzed products are mostly in conformity with standards. The estimation of the oxidative stability revealed that tub margarines are the least stable. Fatty acid composition showed that practically all the analyzed products are rich in SFA. CONCLUSION: Large amounts of highly saturated oils such as palm oil, coconut and palm kernel are used. The fact that the trans fatty acids content is not very high in Algerian margarines and vegetable smen is not due to the legislation applied but to the awareness of few industries and especially to the import price exerted on different types of fat. So it is in view of profitability that industries choose raw material and not in a health concern.
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