Cervical cancer is considered the fourth most common malignancy and the fifth fatal cancer in women, oncogenic Human papillomavirus are considered a primary cause of development of cervical cancer. It has also been suggested that viral coexistence may also accelerate the progression of cervical lesions to cervical cancer. This study aims to study the coinfection of Epstein Bar, Herpesvirus 8 and Hepes simplex type 2 infections in women with cervical cancer with the presence of Human papillomavirus and their correlations with the clinicopathologic characteristics of patients. In this study, 73 samples that tested positive for Human papillomavirus in previous study were used for the detection of Epstein Bar, Herpesvirus 8 and Hepes simplex type 2 in tumor tissue using Polymerase Chain Reaction techniques, and the clinical relevance was analyzed statistically. Of the 73 samples (48%) were infected with Epstein Bar, (24.65%) infected with Herpesvirus 8 However, none of the cases were infected with Hepes simplex type 2. The frequency of co-infections was 16.43% of cases. No significant association was found between co-infection and other clinicopathologic features. Therefore, these results represent arguments in favor of the role of Epstein Bar and Herpesvirus 8 among Human papillomavirus positive cases, as potential cofactors in cervical carcinogenesis, which could lead us to develop new therapeutics and preventive vaccines.

Introduction The IUD is one of the most widely used reversible, long-term contraceptive methods in the world. About 80% of IUDs are found in the peritoneal cavity after uterine perforation. Case report A 27-year-old female patient presented with chronic pelvic pain with minimal metrorrhagia for 8 months on IUD. On examination, there was no IUD thread. Pelvic ultrasound showed a hypoechoic, heterogeneous, poorly limited formation measuring 3 × 2.68 cm. Abdominal-pelvic CT scan showed hyperdense supravesical material surrounded by a hypo-dense, well-limited collection measuring 26 × 25 mm. Laparoscopy showed an anterior peritoneal collection above the bladder containing the IUD, a uterus, adnexa, and a bladder without abnormality. The IUD was removed after incision of the collection and aspiration of the pus. Discussion The IUD is one of the most widely used long-term reversible contraceptive methods in the world. But like any foreign body, it can present complications, notably migration after uterine perforation, which remains rare, and even rarer peritoneal localization. The clinical diagnosis is not always obvious, and additional examinations are necessary to locate the device, including endovaginal ultrasound, a CT scan or magnetic resonance imaging. WHO recommends surgical removal of the migrated IUD by minimally invasive methods, including hysteroscopy, cystoscopy, colonoscopy, or laparoscopy, depending on the location of the IUD. Conclusion IUDs are effective contraceptive measures, and the majority of patients with uterine perforation by IUD migration are asymptomatic. Diagnosis is based on a thorough gynecologic analysis and appropriate radiologic imaging.

Background: The purpose of our case control study is to explore the potential association of tumor protein 53 (TP53) c.215G>C, p. (Arg72Pro) polymorphism (rs1042522) with the risk of breast cancer (BC) development in the Moroccan population. Methods and Results: The study population consisted of 125 female patients with confirmed BC and 126 healthy controls. DNA samples were genotyped by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism assay method using BstUI restriction enzyme. We showed that the homozygous genotype of TP53 72Pro variant was significantly associated with increased BC risk (OR 2.2, 95% CI 1.07–4.54, p = 0.03). The dominant and additive models of TP53 Pro allele were also correlated to the risk of BC (OR 2.13, 95% CI 1.07–4.23, p = 0.02 and OR 1.49, 95% CI 1.03–2.16, p = 0.03, respectively). Furthermore, the TP53 Arg72 variant was associated with protection against BC, either in the homozygous genotype, the dominant or the additive models (OR 0.45, 95% CI 0.22–0.93, p = 0.03; OR 0.46, 95% CI 0.23–0.92, p = 0.029 and OR 0.67, 95% CI 0.46–0.97, p = 0.03, respectively). Conclusion: Our results suggest that TP53 c.215G>C, p. (Arg72Pro) polymorphism may be considered as a genetic marker for predisposition to BC in Moroccan population.

Breast cancer is the most common type of tumor in women worldwide. Immune checkpoint inhibitors, particularly anti-PDL1, have shown promise as a therapeutic approach for managing this disease. However, this type of immunotherapy still fails to work for some patients, leading researchers to explore alternative immune checkpoint targets. The Ig suppressor of T cell activation domain V (VISTA) has emerged as a novel immune checkpoint that delivers inhibitory signals to T cells and has demonstrated encouraging results in various cancers. Our study investigated the association of VISTA expression with clinicopathological parameters in breast cancer patients, its involvement in the Epithelial-Mesenchymal-Transition (EMT) process, and its correlation with PD1 expression. Transcriptomic analysis revealed that VISTA was associated with lobular and metaplastic histological type, tumor size, lymph node status, ER and PR negative status, and the TNBC molecular subtype. Furthermore, VISTA expression was strongly associated with an immunosuppressive tumor microenvironment. Immunohistochemistry analysis corroborated the transcriptomic results, indicating that VISTA was expressed in most immune cells (94%) and was significantly expressed in breast cancer tumor cells compared to matched adjacent tissues. Our study also showed for the first time that VISTA overexpression in breast cancer cells could be associated with the EMT process. Additionally, we identified a positive correlation between VISTA and PD-1 expression. Together, these results highlight the immunosuppressive effect of VISTA in breast cancer patients and suggest that bi-specific targeting of VISTA and PD-1 in combination therapy could be beneficial for these patients.

L'inversion utérine non puerpérale chronique est une situation clinique extrêmement rare, 85% des inversions sont puerpérales exposant l'accouchée au risque d'hémorragie de la délivrance cataclysmique. Nous rapportons le cas d'une patiente de 70 ans qui s'est présentée en consultation pour prolapsus uro-génital du 3 ème degré, une hystérectomie totale sans conservation annexielle a été réalisée par un double abord: voie vaginale complétée par une laparotomie mettant en évidence une inversion utérine à contenu annexielle bilatérale. La voie abdominale a permis en plus d'une bonne exposition chirurgicale, l'exclusion d'un contenu digestif ou urinaire pris dans l'inversion avant la réalisation de l'hystérectomie. Bien que rare et de diagnostic difficile, l'inversion utérine non puerpérale aiguë est une urgence médico-chirurgicale.