Microplastic ingestion by the farmed sea cucumber is undocumented. Microplastics were isolated from the sea cucumber Apostichopus japonicus that was collected from eight farms along the Bohai Sea and the Yellow Sea in China. To examine microplastic ingestion, the intestines were isolated, digested and then subjected to the floatation test. The microplastic abundance in the sediment ranged from 20 to 1040 particles kg À1 of dry sediment, while the ingested microplastics ranged from 0 to 30 particles intestine À1. After filtering the coelomic fluid, the extracted microplastics from the coelomic fluid ranged from 0 to 19 particles animal À1. Thus, we speculated that microplastics may transfer to the coelomic fluid of sea cucumber. The ingested microplastics did not correlate with the animal body weight but was site dependent, suggesting that sea cucumber may serve as sentinel for microplastic pollution monitoring in the sediment. The microplastics were identified by Fourier transform infrared micro spectroscopy, and the polymer types were mainly cellophane, polyester, and polyethylene terephthalate. This study revealed that, microplastics widely existed in sea cucumber farms, and that sea cucumbers ingest microplastics as suitable with their mouth open. Moreover, the microplastics might transfer to the coelomic fluid of the sea cucumber. Further investigations are needed to assess the chronic effect of the microplastics on the growth and physiological status of the sea cucumber.
Thirty-nine volatile compounds identified in hydrodistilled essential oil (HD) of Egyptian parsley, representing 97.87% of the total oil, while solid-phase microextraction (HS-SPME) revealed 16 components constituting 96.54% of the volatile material. Monoterpenes were the predominant in both extracts with considerable quantitative differences, while a dramatic decrease in myristicin from 26.21% in HD extract to 4.87% in HS-SPME extract occurred. Myristicin, β-phellandrene and myrecene were the major components among 38 identified components accounting for 92.87% of the total identified volatiles in Madinah hydrodistillate. Parsley essential oil of Madinah exhibited a higher scavenging ability for 2,2ʹ-diphenyl-1-picrylhydrazyl in comparison to Egyptian parsley oil.
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