This investigation was implemented during two successive seasons (2020 and 2021) on Ruby Seedless grapevines cultivated at the Experimental Orchard of Assiut University, Faculty of Agriculture. This work was designed to investigate the effectiveness of Ascorbic, Citric, and Abscisic acids on yield and anthocyanin concentration in the Ruby Seedless grape cultivar under Assiut climatic conditions. The obtained results revealed that all the treatments significantly increased the total yield/vine as compared to the control (untreated vines). Importantly, the highest yield weight was acquired from the Ascorbic acid (AA) at 600 ppm + Citric acid (CA) at 600 ppm + Abscisic acid (ABA) at 600 ppm treatment. In addition, the treatment of AA at 600 ppm + CA at 600 ppm + ABA at 600 ppm gave the highest value of anthocyanin content.