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River sediments are basic components of our environment. It also constitutes a major source of persistent bioaccumulative toxic chemicals which may pose threats to ecological and human health even after contaminants are no longer released from point and non-point sources. Therefore, the aim of this study was to investigate the mobility and the availability of metals in sediments from different sites along the Nile River in Cairo district using sequential chemical extraction technique. The speciation data showed that most metals were associated with organic/ sulfide and residual fractions. The order of total metal concentrations in sediment samples was found to be Fe [ Mn [ Zn [ Ni [ Cu C Cr [ Pb [ Cd.